| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RELT |
| Uniprot No | Q969Z4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-153aa |
| 氨基酸序列 | STTLWQCPPGEEPDLDPGQGTLCRPCPPGTFSAAWGSSPCQPHARCSLWRRLEAQVGMATRDTLCGDCWPGWFGPWGVPRVPCQPCSWAPLGTHGCDEWGRRARRGVEVAAGASSGGETRQPGNGTRA |
| 预测分子量 | 17.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RELT重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：Structural and functional characterization of recombinant human RELT
**作者**：Smith A, et al.
**摘要**：该研究通过原核表达系统成功制备了重组人RELT蛋白，解析了其胞外结构域的晶体结构，并证实其能够与特定配体结合激活NF-κB信号通路，为研究RELT在免疫调控中的作用提供依据。
2. **文献名称**：RELT recombinant protein modulates T-cell responses in autoimmune models
**作者**：Chen L, et al.
**摘要**：作者利用哺乳动物细胞表达系统获得高纯度RELT-Fc融合蛋白，发现其可通过抑制T细胞过度活化减轻小鼠实验性自身免疫性脑脊髓炎症状，提示其在自身免疫疾病中的治疗潜力。
3. **文献名称**：Expression optimization and functional analysis of RELT extracellular domain in insect cells
**作者**：Wang Y, et al.
**摘要**：研究通过杆状病毒-昆虫细胞系统优化RELT胞外区表达条件，发现重组蛋白能特异性结合巨噬细胞表面受体并诱导促炎因子分泌，揭示了RETL在天然免疫中的潜在调控机制。
注：上述文献为示例性概括，实际文献需通过PubMed、Web of Science等数据库检索获取。建议使用关键词"RELT recombinant protein"、"RELT signaling"进行精准检索。
RELT (Receptor Expressed in Lymphoid Tissues) is a member of the tumor necrosis factor receptor superfamily (TNFRSF), which plays diverse roles in immune regulation, cell survival, and inflammation. Initially identified in activated T cells, RELT is expressed in lymphoid tissues and certain non-immune cells. Its extracellular domain contains cysteine-rich motifs characteristic of TNFRSF members, while its cytoplasmic region lacks canonical death domains, suggesting unique signaling mechanisms.
Recombinant RELT protein is engineered using genetic cloning techniques, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications. This enables researchers to study its structure, ligand interactions, and downstream pathways. RELT’s ligand remains unidentified, but studies suggest it may function as a co-stimulatory receptor or interact with other TNFRSF proteins. It has been implicated in T-cell activation, apoptosis modulation, and inflammatory responses, though its exact biological role remains under investigation.
Interest in RELT stems from its potential therapeutic relevance. Aberrant RELT expression is linked to autoimmune disorders and cancers, making it a candidate for immunotherapy targets. Recombinant RELT proteins are used in vitro and in vivo to explore its signaling crosstalk, particularly with NF-κB and MAPK pathways, and to develop neutralizing antibodies or agonists. Further research aims to clarify its physiological functions and harness its regulatory mechanisms for treating immune-related diseases.
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