| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANAPC5 |
| Uniprot No | Q9UJX4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-232aa |
| 氨基酸序列 | ASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALT |
| 预测分子量 | 53.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANAPC5重组蛋白的3篇代表性文献：
1. **"Structure of the APC5 C-terminal domain from Homo sapiens"**
*作者：Zhang et al. (2016)*
**摘要**：该研究解析了人源ANAPC5亚基C端结构域的晶体结构，揭示了其作为APC/C复合体核心组件的构象特征，并探讨其在底物识别和泛素化调控中的作用。
2. **"Recombinant expression and functional characterization of the anaphase-promoting complex subunit APC5"**
*作者：Cheng et al. (2018)*
**摘要**：通过大肠杆菌和昆虫细胞系统成功表达并纯化重组ANAPC5蛋白，验证其在APC/C复合体组装中的必要性，并证明其缺失导致细胞周期阻滞和有丝分裂异常。
3. **"APC5 mutations disrupt cellular proliferation in colorectal cancer by impairing c-MYC degradation"**
*作者：Wang et al. (2020)*
**摘要**：研究发现结直肠癌中ANAPC5的体细胞突变会破坏APC/C复合体功能，导致c-MYC蛋白异常积累，促进肿瘤细胞增殖，为靶向治疗提供新思路。
4. **"Structural insights into APC/C-mediated substrate recognition by ANAPC5"**
*作者：Barford et al. (2019)*
**摘要**：利用冷冻电镜技术解析了包含ANAPC5的APC/C全复合体结构，阐明其通过特定结构域与底物结合并协调泛素化过程的分子机制。
*注：文献信息为模拟示例，实际引用需核对真实出版物。*
**Background of ANAPC5 Recombinant Protein**
The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase essential for regulating cell cycle progression, particularly during mitosis and the transition from metaphase to anaphase. ANAPC5 (APC5), a core subunit of the APC/C, plays a critical role in maintaining the structural integrity and catalytic activity of the complex. The APC/C orchestrates the ubiquitination of specific target proteins, such as securin and cyclin B, marking them for proteasomal degradation. This process ensures proper chromosome segregation, mitotic exit, and cell cycle phase transitions. Dysregulation of APC/C function is linked to genomic instability, developmental disorders, and cancers.
Recombinant ANAPC5 protein is produced through heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactions with other APC/C subunits (e.g., CDC20 or CDH1 co-activators), and mechanisms underlying substrate recognition. Structural studies reveal that ANAPC5 contains tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions critical for APC/C assembly and activity. Researchers use recombinant ANAPC5 to reconstitute functional APC/C *in vitro*, enabling investigations into its regulation by phosphorylation, inhibitors, or checkpoint signals.
Additionally, ANAPC5 recombinant protein aids in identifying mutations or dysfunctions associated with diseases. For example, mutations in *ANAPC5* have been implicated in intellectual disability and autosomal recessive disorders, highlighting its importance in neurodevelopment. By leveraging recombinant ANAPC5. therapeutic strategies targeting APC/C activity in cancer or genetic disorders can be explored. Overall, this tool is pivotal for advancing understanding of cell cycle control and developing targeted therapies.
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