| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCRFS1 |
| Uniprot No | P47985 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 79-274aa |
| 氨基酸序列 | SHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG |
| 预测分子量 | 48.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCRFS1重组蛋白的3篇示例参考文献(注：文献为示例性质，建议通过学术数据库核实真实文献)：
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1. **标题**: *"Recombinant Expression and Functional Analysis of UQCRFS1 in Mitochondrial Complex III Assembly"*
**作者**: Zhang et al., 2015
**摘要**: 该研究在大肠杆菌中重组表达了UQCRFS1蛋白，验证其在线粒体复合体III组装中的关键作用。实验表明，UQCRFS1缺失导致复合体稳定性下降及电子传递链活性受损。
2. **标题**: *"Structural Role of UQCRFS1 in the Cytochrome bc1 Complex: Insights from Cryo-EM"*
**作者**: Xia et al., 2019
**摘要**: 通过冷冻电镜解析了包含UQCRFS1的人源细胞色素bc1复合体(复合体III)的高分辨率结构，揭示了UQCRFS1与其他亚基的相互作用，及其在维持复合体构象中的必要性。
3. **标题**: *"UQCRFS1 Deficiency Promotes Mitochondrial ROS Generation and Cancer Metastasis"*
**作者**: Lee et al., 2020
**摘要**: 研究利用重组UQCRFS1蛋白进行功能回补实验，发现其缺失导致线粒体活性氧(ROS)水平升高，进而增强肿瘤细胞的侵袭转移能力。
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**建议**：通过PubMed或Google Scholar搜索关键词“UQCRFS1 recombinant”“UQCRFS1 complex III”获取最新文献。真实研究多集中于其结构、功能及与疾病的关联。
UQCRFS1 (Ubiquinol-Cytochrome c Reductase, Rieske Iron-Sulfur Polypeptide 1) is a nuclear-encoded mitochondrial protein that serves as a critical subunit of Complex III (cytochrome bc1 complex) in the electron transport chain (ETC). This complex facilitates electron transfer from ubiquinol to cytochrome c, coupled with proton translocation across the inner mitochondrial membrane, thereby driving ATP synthesis. The UQCRFS1 protein anchors the Rieske iron-sulfur cluster (2Fe-2S), which is essential for the catalytic activity of Complex III. Mutations or dysregulation of UQCRFS1 have been linked to mitochondrial disorders, cancer progression, and neurodegenerative diseases due to disrupted oxidative phosphorylation (OXPHOS) and elevated reactive oxygen species (ROS) production.
Recombinant UQCRFS1 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its structural and functional roles. It enables in vitro analysis of enzyme kinetics, protein-protein interactions within Complex III, and the impact of pathogenic mutations. Researchers utilize this tool to investigate mitochondrial dysfunction mechanisms, screen potential therapeutics targeting ETC deficiencies, and explore UQCRFS1’s regulatory role in apoptosis and metabolic reprogramming. Challenges in producing functional recombinant UQCRFS1 include maintaining proper folding of the iron-sulfur cluster domain and mimicking post-translational modifications critical for its activity. Current studies also focus on its tissue-specific isoforms and involvement in hypoxia adaptation, highlighting its broader implications in cancer metabolism and age-related pathologies.
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