| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTDSS1 |
| Uniprot No | P48651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-35aa |
| 氨基酸序列 | MASCVGSRTLSKDDVNYKMHFRMINEQQVEDITID |
| 预测分子量 | 31.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性示例(非真实文献)：
1. **文献名称**: "Recombinant Expression and Functional Characterization of Human Phosphatidylserine Synthase 1 (PTDSS1)"
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组PTDSS1蛋白，验证其催化磷脂酸转化为磷脂酰丝氨酸的酶活性，为体外研究磷脂代谢机制提供工具。
2. **文献名称**: "Structural Insights into PTDSS1 Catalytic Mechanism via Recombinant Protein Crystallography"
**作者**: Tanaka K, et al.
**摘要**: 通过重组PTDSS1蛋白的结晶与结构解析，揭示了其活性位点关键氨基酸残基的作用，阐明了底物结合与催化的分子机制。
3. **文献名称**: "PTDSS1 Recombinant Protein Modulates Apoptotic Signaling in Cancer Cells"
**作者**: Zhang Y, et al.
**摘要**: 利用重组PTDSS1蛋白处理癌细胞，发现其通过调节细胞膜磷脂酰丝氨酸分布影响凋亡信号通路，提示潜在治疗靶点价值。
4. **文献名称**: "Optimization of PTDSS1 Recombinant Production in Mammalian Expression Systems"
**作者**: Lee J, et al.
**摘要**: 比较哺乳动物表达系统中PTDSS1重组蛋白的产量与活性，优化培养条件以获得高纯度功能性蛋白用于疾病模型研究。
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注：以上内容为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
Phosphatidylserine synthase 1 (PTDSS1) is a key enzyme involved in phospholipid metabolism, specifically catalyzing the synthesis of phosphatidylserine (PS) from phosphatidylcholine or phosphatidylethanolamine via a base-exchange reaction. As an integral membrane protein located in the endoplasmic reticulum, PTDSS1 plays a critical role in maintaining membrane phospholipid asymmetry and cellular homeostasis. Its function is particularly vital for membrane-associated processes, including apoptosis, blood coagulation, and cell signaling. Dysregulation of PTDSS1 has been implicated in various pathologies, such as cancer progression, neurodegenerative disorders, and Lenz-Majewski syndrome—a rare genetic condition linked to gain-of-function mutations in the PTDSS1 gene.
Recombinant PTDSS1 protein is engineered using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to enable detailed biochemical and structural studies. The production typically involves codon optimization, affinity tag fusion (e.g., His-tag), and purification via chromatography to ensure high purity and activity. This recombinant tool has become indispensable for investigating PS biosynthesis mechanisms, drug discovery targeting lipid metabolism disorders, and functional assays to characterize disease-associated mutations. Recent studies also explore its potential in cancer immunotherapy, as PS externalization on tumor cells influences immune evasion. Despite challenges in expressing full-length membrane-bound forms, truncated soluble variants or nanodisc-incorporated PTDSS1 have advanced structural resolution efforts. Ongoing research aims to elucidate its regulatory interactions with lipid transporters and therapeutic relevance in age-related diseases.
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