| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCR11 |
| Uniprot No | O14957 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-56aa |
| 氨基酸序列 | MVTRFLGPRYRELVKNWVPTAYTWGAVGAVGLVWATDWRLILDWVPYINGKFKKDN |
| 预测分子量 | 33.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCR11重组蛋白的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **"Recombinant expression and functional characterization of human UQCR11 in mitochondrial complex III assembly"**
*Author: Zhang L. et al.*
摘要：本研究成功在大肠杆菌中重组表达了人源UQCR11蛋白，并证明其在线粒体复合体III组装中的关键作用。通过体外功能实验发现，UQCR11缺失会导致复合体III活性下降，提示其在电子传递链中的重要性。
2. **"Structural insights into UQCR11 interactions within the cytochrome bc1 complex"**
*Author: Müller S. & Schmidt O.*
摘要：利用X射线晶体学解析了UQCR11与其结合伴侣UQCR10的复合物结构，揭示了二者在维持复合体III稳定性中的相互作用界面，为相关遗传性线粒体疾病的机制研究提供依据。
3. **"UQCR11 overexpression promotes tumor growth via ROS-mediated signaling pathways"**
*Author: Tanaka K. et al.*
摘要：通过重组UQCR11蛋白在癌细胞中的过表达实验，发现其通过调控活性氧(ROS)水平激活MAPK通路，促进肿瘤细胞增殖，提示UQCR11可能成为癌症治疗新靶点。
注：以上文献信息为示例性虚构内容，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。
**Background of UQCR11 Recombinant Protein**
UQCR11 (Ubiquinol-cytochrome c reductase complex subunit 11) is a nuclear DNA-encoded component of mitochondrial Complex III (cytochrome *bc*₁ complex), a critical enzyme in the electron transport chain (ETC) responsible for ATP synthesis. This protein plays a role in stabilizing Complex III and maintaining its functional integrity, facilitating electron transfer from ubiquinol to cytochrome *c* while contributing to proton gradient generation across the mitochondrial inner membrane. Dysregulation of UQCR11 has been linked to mitochondrial disorders, metabolic imbalances, and cancer progression due to its involvement in cellular energy metabolism and redox regulation.
The recombinant UQCR11 protein is produced via genetic engineering, often expressed in *E. coli* or mammalian cell systems, enabling studies on its structure, interactions, and mechanistic roles. Its recombinant form retains key functional domains, including conserved regions critical for binding to other Complex III subunits (e.g., UQCRB, UQCRC1). Researchers utilize this protein to investigate mitochondrial dysfunction, oxidative stress responses, and diseases like Leigh syndrome or colorectal cancer, where UQCR11 expression is frequently altered.
Recent studies highlight UQCR11’s potential as a therapeutic target, particularly in cancers reliant on oxidative phosphorylation. Its recombinant variant also aids in drug screening, antibody development, and structural analyses via techniques like X-ray crystallography or cryo-EM. Overall, UQCR11 recombinant protein serves as a vital tool for unraveling mitochondrial biology and advancing precision medicine for metabolic and degenerative diseases.
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