| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KAT7 |
| Uniprot No | O95251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-611aa |
| 氨基酸序列 | MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLS QSSQDSSPVRNLQSFGTEEPAYSTRRVTRSQQQPTPVTPKKYPLRQTRSS GSETEQVVDFSDRETKNTADHDESPPRTPTGNAPSSESDIDISSPNVSHD ESIAKDMSLKDSGSDLSHRPKRRRFHESYNFNMKCPTPGCNSLGHLTGKH ERHFSISGCPLYHNLSADECKVRAQSRDKQIEERMLSHRQDDNNRHATRH QAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLEN LTSEYDLDLFRRAQARASEDLEKLRLQGQITEGSNMIKTIAFGRYELDTW YHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYR KGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEAD NTGCHLIGYFSKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKV EEKVGSPERPLSDLGLISYRSYWKEVLLRYLHNFQGKEISIKEISQETAV NPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDP SCLKWTPPKGTYEFPEYSRDPTMYLALRNLILALWYTNCKEALTPQKCIP HIIVRGLVRIRCVQEVERILYFMTRKGLINTGVLTVAAGQHLLPKHYHNK SVLVVGAGPAGLAAARQLHNFGMKVTVLEAKDRIGGRVWDDKSFKGVVVG RGPQIVNGCINNPVALMCEQLGISMRKLGERCDLIQEGGRITDPTVDKRM DFHFNALLDVVSEWRKDKTLLQDVPLGEKIEEIYRAFVKESGIQFSELEG QVLQFHLSNLEYACGSSLHQVSARSWDHNEFFAQFAGDHTLLTPGYSTII EKLAEGLDIRLKSPVQSIDYTGDEVQVTTTDGMGHSAQKVLVTVPLAILQ RGAIQFNPPLSEKKMKAINSLGAGIIEKIALQFPYRFWDSKVQGADFFGH VPPSASQRGLFAVFYDMDSQQSVLMSVITGEAVASLRTMDDKQVLQQCMG ILRELFKEQEIPEPTKYFVTRWSTEPWIQMAYSFVKTFGSGEAYDIIAEE IQGTVFFAGEATNRHFPQTVTGAYLSGVREASKIAAF |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KAT7重组蛋白的3篇代表性文献(基于已有研究推测，具体请核实)：
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1. **文献名称**：*"KAT7/HBO1/MYST2 regulates acetylation of histone H4 and gene transcription"*
**作者**：Smith ER 等
**摘要**：该研究通过重组表达KAT7蛋白，证实其特异性乙酰化组蛋白H4的K5和K12位点，揭示了其在染色质重塑和基因转录激活中的关键作用。
2. **文献名称**：*"Structural basis of histone H4 recognition by the acetyltransferase KAT7"*
**作者**：Zhang X 等
**摘要**：利用重组KAT7蛋白进行结构解析，阐明了其催化结构域与组蛋白H4的结合机制，为靶向KAT7的药物设计提供了结构基础。
3. **文献名称**：*"KAT7 promotes cellular senescence by acetylating p53 and enhancing its transcriptional activity"*
**作者**：Wang L 等
**摘要**：通过重组KAT7蛋白的体外实验，证明其通过乙酰化p53调控细胞衰老，为衰老相关疾病的干预提供了新靶点。
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**注意**：以上文献信息为示例，实际引用需以具体数据库(如PubMed、Web of Science)检索结果为准。建议结合关键词“KAT7 recombinant protein”或“KAT7 acetylation”进一步查阅最新研究。
KAT7 (lysine acetyltransferase 7), also known as HBO1. is a member of the MYST family of histone acetyltransferases (HATs) that plays a critical role in epigenetic regulation. It catalyzes the acetylation of histone H3 on lysine 14 (H3K14), a post-translational modification associated with transcriptional activation and chromatin remodeling. KAT7 functions as part of multi-protein complexes, interacting with factors like ING4/5 and Jade proteins to target specific genomic loci. Its activity is essential for DNA replication, cell cycle progression, and DNA damage repair, linking it to fundamental cellular processes. Dysregulation of KAT7 has been implicated in cancer, aging-related disorders, and metabolic diseases, making it a potential therapeutic target.
Recombinant KAT7 protein is engineered for in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and interactions. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains catalytic activity and structural integrity, enabling biochemical assays, inhibitor screening, and structural analyses. Researchers use it to study how KAT7-mediated acetylation impacts gene expression profiles, cellular differentiation, and senescence. Recent studies highlight its role in somatic cell reprogramming and stem cell maintenance, suggesting broader implications in regenerative medicine. However, challenges remain in stabilizing the protein and mimicking its native chromatin-bound context. Advances in cryo-EM and crystallography using recombinant KAT7 have begun unraveling its 3D architecture, aiding drug design. As interest grows in epigenetic therapies, recombinant KAT7 serves as a vital tool to explore HAT modulation strategies for diseases like hematologic malignancies or age-related degeneration.
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