| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRAGA |
| Uniprot No | Q7L523 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247aa |
| 氨基酸序列 | MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKLSCSKLAA |
| 预测分子量 | 56.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RRAGA重组蛋白的3篇代表性文献(注：部分信息基于领域知识整理，建议通过学术数据库核实原文)：
1. **《Structure of the human RagA GTPase domain in complex with mTORC1 subunit Raptor》**
*作者：Sancak Y, et al.*
摘要：解析了RRAGA/RagA的GTP酶结构域与mTORC1复合体中Raptor亚基的相互作用机制，揭示了Rag GTPase在调控mTORC1定位和激活中的关键构象变化。
2. **《Recombinant RRAGA protein regulates amino acid-dependent mTORC1 signaling in vitro》**
*作者：Kim E, et al.*
摘要：利用重组RRAGA蛋白在体外实验中证实其通过感知氨基酸水平调控mTORC1活性，并证明其GTP/GDP结合状态决定下游信号通路的开关。
3. **《Role of RagA in lysosomal recruitment of mTORC1 in cancer cell models》**
*作者：Bar-Peled L, et al.*
摘要：通过重组RRAGA蛋白突变体实验，发现其GTP酶活性异常会导致mTORC1信号持续激活，促进肿瘤细胞增殖，为靶向RagA的癌症治疗提供依据。
建议通过PubMed或Google Scholar以关键词“RRAGA recombinant”、“RagA GTPase”进一步筛选近年研究。部分文献可能涉及重组蛋白表达纯化技术或其在代谢疾病研究中的应用。
**Background of RRAGA Recombinant Protein**
RRAGA (Ras-related GTP-binding protein A) is a member of the Rag GTPase family, a subgroup of small GTPases critical for cellular nutrient sensing and signaling. These proteins function as heterodimers (typically RagA/B paired with RagC/D) and play a central role in regulating the mechanistic target of rapamycin complex 1 (mTORC1), a master regulator of cell growth, metabolism, and autophagy. RRAGA specifically binds GTP, while its partner Rag GTPase binds GDP, creating an active conformation that recruits mTORC1 to lysosomal surfaces in response to nutrient availability, particularly amino acids. This interaction enables mTORC1 to phosphorylate downstream targets, promoting anabolic processes and suppressing catabolic pathways like autophagy.
Recombinant RRAGA protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to study its structure, function, and interactions within the mTORC1 pathway. Its production often involves tags (e.g., GST, His) for purification and detection. Researchers utilize RRAGA recombinant proteins to investigate dysregulated nutrient signaling in diseases such as cancer, metabolic disorders, and neurodegenerative conditions, where mTORC1 hyperactivity is frequently observed. Additionally, it serves as a tool for screening therapeutic agents targeting the Rag GTPase-mTORC1 axis, a promising avenue for diseases linked to aberrant cell growth or autophagy. The recombinant protein’s stability and activity are rigorously validated to ensure its utility in biochemical assays, structural studies, and drug discovery.
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