| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGD |
| Uniprot No | P52209 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PGD(磷酸葡萄糖脱氢酶)重组蛋白研究的模拟参考文献示例(文献信息为虚构，仅作格式参考)：
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1. **文献名称**: "High-yield expression and purification of recombinant human 6-phosphogluconate dehydrogenase in Escherichia coli"
**作者**: Chen, L., & Wang, H.
**摘要**: 本研究报道了一种优化大肠杆菌表达系统生产重组人源6-磷酸葡萄糖脱氢酶(6PGD)的方法，通过密码子优化和诱导条件调控显著提高蛋白产量，并验证了纯化后酶的催化活性，为后续代谢研究提供工具。
2. **文献名称**: "Structural insights into 6PGD-mediated cancer metabolism and inhibitor development"
**作者**: Martinez, R., et al.
**摘要**: 通过X射线晶体学解析了重组6PGD蛋白的三维结构，揭示了其底物结合位点及与肿瘤代谢异常的关系，并基于结构筛选了小分子抑制剂，为靶向癌症治疗的药物设计提供依据。
3. **文献名称**: "Functional characterization of recombinant PGD in redox homeostasis and oxidative stress response"
**作者**: Kim, S., et al.
**摘要**: 利用哺乳动物细胞表达重组PGD蛋白，证明其在维持NADPH/NADP+平衡和抵抗氧化应激中的关键作用，揭示了PGD缺陷与代谢疾病的潜在关联。
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**说明**：以上文献为示例性内容，实际引用需检索PubMed、Web of Science等数据库获取真实文献(关键词：6-phosphogluconate dehydrogenase, recombinant protein, PGD expression)。
**Background of PGD Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, are pivotal tools in biomedical research and therapeutic development. Among these, PGD (Prostaglandin D) recombinant proteins have garnered attention due to their role in regulating inflammation, immune responses, and cellular homeostasis. PGD is a lipid mediator derived from arachidonic acid via cyclooxygenase (COX) enzymes, with two isoforms: PGD₁ and PGD₂. PGD₂, in particular, is synthesized by hematopoietic prostaglandin D synthase (H-PGDS) and lipocalin-type prostaglandin D synthase (L-PGDS), playing critical roles in allergic responses, sleep regulation, and tumor suppression.
The production of PGD recombinant proteins leverages recombinant DNA technology, where target genes encoding PGD synthases or receptors are cloned into expression vectors (e.g., bacterial, yeast, or mammalian systems). This enables scalable, high-purity protein production for functional studies. Mammalian systems are often preferred to ensure proper post-translational modifications and biological activity.
PGD recombinant proteins are widely used to dissect signaling pathways, particularly interactions with DP1 and DP2 receptors, which mediate pro-inflammatory and anti-inflammatory effects. They also aid in drug discovery, such as developing inhibitors for H-PGDS in asthma or L-PGDS in neurodegenerative diseases. Additionally, these proteins serve as standards in diagnostic assays to quantify PGD levels in pathological conditions.
Challenges include maintaining protein stability and mimicking native conformational states. Advances in structural biology and protein engineering, such as site-directed mutagenesis or fusion tags, have improved functionality and yield. As research unravels the dual roles of PGD in promoting/resolving inflammation, recombinant PGD proteins remain essential for therapeutic innovation and understanding complex disease mechanisms.
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