| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2CB |
| Uniprot No | P62714 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309aa |
| 氨基酸序列 | MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL |
| 预测分子量 | 59 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP2CB重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**：*Structure of the protein phosphatase 2A holoenzyme*
**作者**：Xu, Y., Chen, Y., Zhang, P., et al.
**摘要**：该研究解析了PP2A全酶的冷冻电镜结构，明确了PPP2CB(催化亚基)与其他调节亚基的相互作用，揭示了其底物识别和酶活性调控的分子机制。
2. **文献名称**：*Recombinant expression and functional characterization of the PP2A catalytic subunit in Saccharomyces cerevisiae*
**作者**：Evans, D.R.H., Myles, T., Hofsteenge, J.
**摘要**：报道了在酵母中重组表达PPP2CB的方法，验证了其磷酸酶活性，并分析了突变对功能的影响，为研究PP2A的调控机制提供了工具。
3. **文献名称**：*PP2A holoenzyme assembly: in cauda venenum (the sting is in the tail)*
**作者**：Janssens, V., Goris, J.
**摘要**：综述了PP2A全酶(含PPP2CB)的组装过程，强调重组蛋白技术在研究亚基互作和疾病相关突变中的关键作用，如癌症和神经退行性疾病。
(注：上述文献为领域内代表性研究，具体细节建议通过PubMed或Sci-Hub进一步检索确认。)
**Background of PPP2CB Recombinant Protein**
PPP2CB, a subunit of the serine/threonine protein phosphatase 2A (PP2A) complex, plays a critical role in regulating cellular signaling pathways by dephosphorylating key substrates. As the catalytic C-subunit of PP2A, PPP2CB is essential for maintaining cellular homeostasis, influencing processes such as cell cycle progression, apoptosis, DNA damage response, and metabolic regulation. The PP2A holoenzyme typically consists of a structural scaffold (A-subunit), a regulatory B-subunit, and the catalytic C-subunit (PPP2CB or PPP2CA), with PPP2CB being ubiquitously expressed across tissues.
Recombinant PPP2CB protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a highly purified, functional form of the enzyme for research. Its recombinant form retains the conserved structural features of the native protein, including the catalytic metal-binding sites (Mn²⁺/Fe³⁺) and active residues critical for phosphatase activity. This allows researchers to study PP2A's enzymatic mechanisms, substrate interactions, and regulatory networks in vitro.
PPP2CB dysfunction is linked to cancers, neurodegenerative diseases, and metabolic disorders, making its recombinant protein a valuable tool for drug discovery and mechanistic studies. It is widely used in kinase-phosphatase activity assays, structural studies (e.g., X-ray crystallography), and screening for therapeutic modulators. Additionally, recombinant PPP2CB aids in exploring post-translational modifications (e.g., methylation, phosphorylation) that regulate PP2A assembly and specificity. By enabling precise biochemical and functional analyses, PPP2CB recombinant protein contributes to understanding PP2A's role in health and disease, offering potential pathways for targeted therapies.
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