| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SALL2 |
| Uniprot No | Q9Y467 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MSRRKQRKPQQLISDCEGPSASENGDASEEDHPQVCAKCCAQFTDPTEFLAHQNACSTDPPVMVIIGGQENPNNSSASSEPRPEGHNNPQVMDTEHSNPPDSGSSVPTDPTWGPERRGEESPGHFLVAATGTAAGGGGGLILASPKLGATPLPPESTPAPPPPPPPPPPPGVGSGHLNIPLILEELRVLQQRQIHQMQ |
| 预测分子量 | 25.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SALL2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Functional characterization of recombinant SALL2 protein in transcriptional repression*
**作者**：Li Y, et al.
**摘要**：研究通过在大肠杆菌中表达并纯化SALL2重组蛋白，发现其通过N端结构域结合特定DNA序列，并在体外实验中抑制靶基因启动子活性，提示其在表观遗传调控中的作用。
2. **文献名称**：*SALL2 recombinant protein expression and its role in ovarian cancer cell apoptosis*
**作者**：Garcia M, et al.
**摘要**：利用哺乳动物细胞系统表达SALL2重组蛋白，发现其过表达诱导卵巢癌细胞凋亡，可能与p53通路相互作用，为癌症治疗提供潜在靶点。
3. **文献名称**：*Structural insights into SALL2 zinc finger domains using recombinant protein crystallography*
**作者**：Chen X, et al.
**摘要**：通过昆虫细胞表达SALL2重组蛋白并进行X射线晶体学分析，揭示其锌指结构域的DNA结合模式，为理解其突变导致发育疾病的机制提供结构基础。
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(注：上述文献为示例性概括，实际引用需根据真实文献调整。)
SALL2. a member of the Spalt-like (SALL) transcription factor family, is characterized by its zinc finger domains that mediate DNA binding and protein interactions. It plays critical roles in embryonic development, cell differentiation, and tissue homeostasis. SALL2 is notably involved in regulating gene networks tied to neurogenesis, eye development, and kidney formation. Studies also link SALL2 to cancer biology, where it exhibits dual roles—acting as a tumor suppressor in some contexts (e.g., ovarian cancer) while promoting tumorigenesis in others (e.g., glioblastoma). Its expression is frequently dysregulated in malignancies, often correlating with clinical outcomes.
Recombinant SALL2 protein, produced via expression systems like *E. coli* or mammalian cells, retains the functional domains necessary for studying its molecular mechanisms. Researchers use it to investigate SALL2's interaction partners, DNA-binding specificity, and regulatory effects on downstream targets such as *p21* or *MYC*. Purification tags (e.g., His-tag) enable efficient isolation for *in vitro* assays. Notably, SALL2 recombinant proteins aid in exploring its role in oxidative stress responses and DNA repair pathways, which may inform therapeutic strategies. Mutations in *SALL2* are associated with developmental disorders, making recombinant variants valuable for modeling genetic defects. Despite progress, functional redundancy within the SALL family and context-dependent signaling pose challenges in delineating SALL2-specific pathways. Current research focuses on its epigenetic regulatory functions and potential as a biomarker or drug target.
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