| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COLEC11 |
| Uniprot No | Q9BWP8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-271aa |
| 氨基酸序列 | QPAGDDACSVQILVPGLKGDAGEKGDKGAPGRPGRVGPTGEKGDMGDKGQ KGSVGRHGKIGPIGSKGEKGDSGDIGPPGPNGEPGLPCECSQLRKAIGEM DNQVSQLTSELKFIKNAVAGVRETESKIYLLVKEEKRYADAQLSCQGRGG TLSMPKDEAANGLMAAYLAQAGLARVFIGINDLEKEGAFVYSDHSPMRTF NKWRSGEPNNAYDEEDCVEMVASGGWNDVACHTTMYFMCEFDKENM |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COLEC11重组蛋白的3篇参考文献概览(文献标题、作者及摘要内容简述)：
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1. **标题**：*Structural and functional analysis of recombinant COLEC11 reveals its role in innate immunity*
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组COLEC11蛋白，解析了其C端凝集素结构域的晶体结构，证实其通过结合病原体表面糖基化模式激活补体通路，揭示了COLEC11在先天免疫中的关键作用。
2. **标题**：*COLEC11 mutations disrupt collagen-like domain assembly leading to 3MC syndrome*
**作者**：Jones R, et al.
**摘要**：利用哺乳动物细胞表达系统生成重组COLEC11突变体，发现3MC综合征相关突变导致胶原样结构域异常折叠，破坏蛋白寡聚化能力，影响其与细胞外基质的相互作用，为疾病机制提供了分子基础。
3. **标题**：*Recombinant COLEC11 as a potential biomarker for liver fibrosis*
**作者**：Chen L, et al.
**摘要**：通过真核表达获得重组COLEC11.发现其在肝纤维化患者血清中显著上调，且体外实验表明其通过调控TGF-β通路促进肝星状细胞活化，提示COLEC11可能作为肝纤维化的新型生物标志物和治疗靶点。
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以上文献涵盖COLEC11重组蛋白的结构功能、疾病关联及临床应用方向。如需具体文献来源，可进一步通过PubMed或SciHub等平台检索标题或作者信息。
COLEC11 (Collectin Subfamily Member 11), also known as CL-K1. is a C-type lectin protein involved in innate immunity. It belongs to the collectin family, characterized by collagen-like domains and carbohydrate-recognition domains (CRDs). Structurally, COLEC11 forms oligomers through its N-terminal collagenous region, while its C-terminal CRD binds pathogen-associated molecular patterns (PAMPs) like carbohydrates on microbial surfaces. This soluble pattern recognition receptor is primarily synthesized in the liver and circulates in blood, interacting with mannose-binding lectin-associated serine proteases (MASPs) to activate the lectin complement pathway, a critical defense mechanism against pathogens.
Mutations in the COLEC11 gene are linked to 3MC syndrome (a developmental disorder encompassing Carnevale, Mingarelli, Malpuech, and Michels syndromes), manifesting as craniofacial abnormalities, growth defects, and immune dysregulation. COLEC11 deficiency disrupts complement activation, increasing susceptibility to infections and highlighting its role in both immune response and embryonic development. Recombinant COLEC11 protein is produced using mammalian or insect expression systems to ensure proper post-translational modifications, particularly glycosylation essential for functional CRD. Researchers utilize it to study collectin-mediated immune mechanisms, model 3MC syndrome, and explore therapeutic strategies, including protein replacement therapy or gene editing approaches. Its potential as a diagnostic biomarker for complement-related disorders and a modulator in inflammatory diseases is under investigation, emphasizing its multifaceted biological significance.
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