| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ORF2 |
| Uniprot No | Q81871 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660aa |
| 氨基酸序列 | MRPRPILLLLLMFLPMLPAPPPGQPSGRRRGRRSGGSGGGFWGDRADSQPFAIPYIHPTNPFAPDVTAAAGAGPRVRQPARPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLSTSPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVVRATIRYRPLVPNAVGGYAISISFWPQTTTTPTSVDMNSITSTDVRILVQPGIASEHVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIHGSLVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFMKDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQQDKGIAIPHDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTTQQYSKTFFVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLAPHSALALLEDTMDYPARAHTFDDFCPECRPLGLQGCAFQSTVAELQRLKMKVGKTREL |
| 预测分子量 | 70,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ORF2重组蛋白的3篇参考文献概览(基于公开研究总结，具体文献请通过学术数据库核实)：
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1. **文献名称**: *Structural analysis of hepatitis E virus ORF2 protein reveals a conserved neutralizing epitope*
**作者**: Li, S., et al.
**摘要**: 研究解析了戊型肝炎病毒(HEV)ORF2重组蛋白的晶体结构，发现其衣壳蛋白上存在高度保守的中和表位，为疫苗设计提供了结构基础。
2. **文献名称**: *Expression and characterization of the ORF2 protein from hepatitis E virus in baculovirus-insect cells*
**作者**: Meng, J., et al.
**摘要**: 报道利用杆状病毒-昆虫细胞系统高效表达HEV ORF2重组蛋白，证明其具有天然构象和强免疫原性，适用于疫苗开发。
3. **文献名称**: *ORF2 recombinant antigen from hepatitis E virus as a target for diagnostic tests*
**作者**: Shukla, P., et al.
**摘要**: 评估HEV ORF2重组蛋白在血清学检测中的特异性与敏感性，提出其作为诊断试剂的核心抗原潜力。
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注：上述内容为示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“ORF2 recombinant protein”+病毒名称(如HEV/HAV)获取。
ORF2 recombinant protein is a key component derived from the open reading frame 2 (ORF2) of various viruses, most notably hepatitis E virus (HEV) and certain retrotransposons like LINE-1. In HEV, ORF2 encodes the viral capsid protein, which is critical for virion assembly, host cell attachment, and immune response modulation. The recombinant form of ORF2 is commonly expressed in heterologous systems (e.g., E. coli, insect, or mammalian cells) for research and diagnostic applications. Its structural integrity enables the formation of virus-like particles (VLPs), mimicking native virions while lacking infectious genetic material. This property has made ORF2 recombinant protein a cornerstone in HEV serological assays, vaccine development (e.g., the HEV 239 vaccine), and studies on viral entry and neutralization.
In retrotransposons, ORF2 encodes proteins with reverse transcriptase and endonuclease activities, essential for genomic integration. Recombinant ORF2 proteins from LINE-1 elements are used to study retrotransposition mechanisms, genome evolution, and their roles in diseases like cancer. The protein’s enzymatic functions are also explored for biotechnological tools, such as targeted DNA insertion.
ORF2 recombinant proteins are characterized via techniques like X-ray crystallography and cryo-EM, revealing conserved domains and epitopes. Challenges include maintaining post-translational modifications in expression systems and optimizing stability for therapeutic use. Ongoing research focuses on enhancing immunogenicity for vaccines, improving diagnostic specificity, and understanding ORF2’s interactions with host factors, contributing to antiviral strategies and genomic research.
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