| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHX9 |
| Uniprot No | Q08211 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 325-840aa |
| 氨基酸序列 | NQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELD |
| 预测分子量 | 74.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DHX9重组蛋白的模拟参考文献示例，基于典型研究领域和结构概括：
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1. **文献名称**: "Recombinant Human DHX9 Helicase: Purification and Biochemical Characterization"
**作者**: Smith A, et al.
**摘要**: 本研究描述了人源DHX9重组蛋白在大肠杆菌中的表达与纯化，并分析了其ATP酶和解旋酶活性。通过体外实验证实了DHX9对双链RNA/DNA底物的解链能力，为后续功能研究提供了基础。
2. **文献名称**: "Structural Insights into the RNA-Binding Domains of DHX9 Using Recombinant Protein Crystallography"
**作者**: Patel R, et al.
**摘要**: 利用重组DHX9蛋白的X射线晶体学，揭示了其N端双链RNA结合域的三维结构，阐明了该区域在核酸识别及病毒RNA相互作用中的关键作用。
3. **文献名称**: "DHX9 Interactions with Viral Proteins: A Study Using Recombinant DHX9 and Co-Immunoprecipitation Assays"
**作者**: Lee J, et al.
**摘要**: 通过重组DHX9蛋白与HIV-1 Tat蛋白的共沉淀实验，证明了二者直接结合，并揭示了DHX9在病毒复制周期中通过调控病毒RNA加工促进感染的机制。
4. **文献名称**: "Role of Recombinant DHX9 in Innate Immune Sensing of Cytosolic DNA"
**作者**: Garcia M, et al.
**摘要**: 本研究利用重组DHX9蛋白，发现其通过识别宿主细胞质DNA并激活cGAS-STING通路，在抗病毒先天免疫应答中发挥重要作用。
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**注**：以上文献为模拟示例，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实文献，并核对作者、标题及摘要准确性。
**Background of DHX9 Recombinant Protein**
DHX9. also known as RNA helicase A (RHA) or Nuclear DNA Helicase II (NDH II), is a multifunctional enzyme belonging to the DEAH-box helicase family. It plays critical roles in various nucleic acid metabolic processes, including transcription, RNA splicing, translation, genomic stability maintenance, and viral replication. DHX9 unwinds DNA-RNA hybrids and secondary structures in RNA, facilitating interactions between nucleic acids and proteins. Its involvement in essential cellular pathways, such as the resolution of R-loops and modulation of innate immune responses, underscores its biological significance.
Structurally, DHX9 contains conserved helicase domains, a double-stranded RNA-binding domain, and nuclear localization signals. Recombinant DHX9 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical activities, structural features, and interactions with nucleic acids or partner proteins. Purification often involves affinity tags (e.g., His-tag) and chromatographic techniques.
Research on recombinant DHX9 has revealed its dual roles in cancer—acting as both a tumor suppressor (by maintaining genome integrity) and an oncogene (by promoting pro-survival signaling). It also interacts with viral RNAs, aiding replication of viruses like HIV and HCV, making it a potential antiviral target. In neurodegenerative diseases, DHX9 dysregulation is linked to abnormal RNA processing.
The availability of recombinant DHX9 enables mechanistic studies, drug screening, and exploration of therapeutic strategies targeting its helicase activity or protein interactions, highlighting its broad relevance in molecular biology and medicine.
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