| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Efemp1 |
| Uniprot No | Q12805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MLKALFLTMLTLALVKSQDTEETITYTQCTDGYEWDPVGQQCKDIDECDI VPDACKGGMKCVNHYGGYLCLPKTAQIIVNNEQPQQETQPAEGTSGATTG VVAASSMATSGVLPGGGFVASAAAVAGPEMQTGRNNFVIRRNPADPQRIP SNPSHRIQCAAGYEQSEHNVCQDIDECTAGTHNCRADQVCINLRGSFACQ CPPGYQKRGEQCVDIDECTIPPYCHQRCVNTPGSFYCQCSPGFQLAANNY TCVDINECDASNQCAQQCYNILGSFICQCNQGYELSSDRLNCEDIDECRT SSYLCQYQCVNEPGKFSCMCPQGYQVVRSRTCQDINECETTNECREDEMC WNYHGGFRCYPRNPCQDPYILTPENRCVCPVSNAMCRELPQSIVYKYMSI RSDRSVPSDIFQIQATTIYANTINTFRIKSGSENGEFYLRQTSPVSAMLV LVKSLSGPREHIVDLEMLTASSIGTFRTSSVLRLTIIVGPFSF |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于EFEMP1重组蛋白的文献摘要信息，供参考：
1. **"EFEMP1 suppresses malignant glioma growth and exerts its action within the tumor extracellular compartment"**
- *Author(s): Hu Y et al.*
- 摘要：研究利用重组EFEMP1蛋白验证其在神经胶质瘤中的抑癌功能，发现其通过调控细胞外基质信号通路抑制肿瘤生长。
2. **"Recombinant EFEMP1 inhibits angiogenesis via blockade of VEGF receptor 2 signaling"**
- *Author(s): Seeliger H et al.*
- 摘要：通过大肠杆菌系统表达重组EFEMP1蛋白，证实其通过拮抗VEGFR2磷酸化抑制血管生成，为抗肿瘤治疗提供潜在靶点。
3. **"Structural and functional analysis of fibulin-3 (EFEMP1) in ocular drusen formation"**
- *Author(s): Marmorstein AD et al.*
- 摘要：利用哺乳动物细胞表达重组EFEMP1蛋白，揭示其异常折叠导致AMD(年龄相关性黄斑变性)中玻璃膜疣沉积的分子机制。
4. **"EFEMP1 enhances cellular resistance to oxidative stress through integrin-mediated PI3K/AKT activation"**
- *Author(s): Kim J et al.*
- 摘要：研究通过纯化重组EFEMP1蛋白，证明其通过与整合素β1结合激活PI3K/AKT通路，提升细胞抗氧化应激能力。
注：以上内容综合了EFEMP1重组蛋白在疾病模型、结构功能及信号通路中的研究，实际文献需通过PubMed/Google Scholar检索标题或作者验证。
**Background of EFEMP1 Recombinant Protein**
EFEMP1 (EGF-containing fibulin-like extracellular matrix protein 1), also known as fibulin-3. is a secreted glycoprotein belonging to the fibulin family, characterized by calcium-binding domains and tandem epidermal growth factor (EGF)-like motifs. It plays a critical role in extracellular matrix (ECM) organization, cell adhesion, and tissue homeostasis, particularly in elastic fibers and basement membranes. EFEMP1 interacts with ECM components like collagen, elastin, and integrins, influencing cellular signaling pathways involved in proliferation, migration, and apoptosis.
Notably, EFEMP1 is linked to several pathological conditions. Mutations in the *EFEMP1* gene are associated with Doyne honeycomb retinal dystrophy (DHRD), a hereditary form of macular degeneration, and age-related macular degeneration (AMD). These mutations disrupt protein folding, leading to abnormal ECM deposition and drusen formation in the retina. EFEMP1 is also implicated in tumor biology, exhibiting dual roles: it suppresses tumor growth in some cancers (e.g., glioma) by inhibiting angiogenesis, yet promotes invasiveness in others (e.g., pancreatic cancer) through matrix metalloproteinase regulation.
Recombinant EFEMP1 protein, produced via expression systems like mammalian or insect cells, retains post-translational modifications crucial for its bioactivity. It is widely used in *in vitro* and *in vivo* studies to investigate ECM dynamics, disease mechanisms, and therapeutic strategies. Researchers utilize EFEMP1 recombinant protein to explore its potential as a biomarker, therapeutic target, or agent for tissue regeneration, particularly in ocular and cancer-related studies. Its structural and functional versatility makes it a key molecule in understanding ECM-related pathologies.
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