| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC41 |
| Uniprot No | Q9Y592 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MLIDERLRCEHHKANYQTLKAEHTRLQNEHVKLQNELKHLFNEKQTQQEKLQLLLEELRGELVEKTKDLEEMKLQILTPQKLELLRAQIQQELETPMRERFRNLDEEVEKYRAVYNKLRYEHTFLKSEFEHQKEEYARILDEGKIKYESEIARLEEDKEELRNQLLNVDLTKDSKRVEQLAREKVYLCQKLKGLEAEVAELKAEKENSEAQVENAQRIQVRQLAEMQATVRSLEAEKQSANLRAERLEKELQSSSEQNTFLINKLHKAEREINTLSSKVKELKHSNKLEITDIKLETARAKSELERERNKIQSELDGLQSDNEILKAAVEHHKVLLVEKDRELIRKVQAAKEEGYQKLVVLQDEKLELENRLADLEKMKVEHDVWRQSEKDQYEEKLRASQMAEEITRKELQSVRLKLQQQIVTIENAEKEKNENSDLKQQISSLQIQVTSLAQSENDLLNSNQMLKEMVERLKQECRNFRSQAEKAQLEAEKTLEEKQIQWLEEKHKLHERITDREEKYNQAKEKLQRAAIAQKKLEQDLELGCPSVTDTYRESVFPPPPLKRDLLK |
| 预测分子量 | 83.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Characterization of CCDC41 Recombinant Protein in Ciliogenesis"**
*Author: Smith J, et al.*
摘要：研究通过重组CCDC41蛋白表达，揭示其在纤毛形成中的调控作用，证实其与微管组织中心的相互作用。
2. **"Functional Analysis of Recombinant CCDC41 in Cell Cycle Progression"**
*Author: Lee H, et al.*
摘要：利用大肠杆菌表达重组CCDC41.发现其磷酸化修饰影响细胞周期G2/M期转换，提示潜在肿瘤治疗靶点。
3. **"CCDC41 Recombinant Protein Interaction Network in Sperm Flagella"**
*Author: Zhang R, et al.*
摘要：通过酵母双杂交筛选，鉴定重组CCDC41与精子鞭毛结构蛋白的互作，支持其在男性不育症中的功能缺失假说。
4. **"Structural Insights into CCDC41 via Recombinant Protein Crystallography"**
*Author: Tanaka K, et al.*
摘要：解析重组CCDC41蛋白的晶体结构，揭示其卷曲螺旋域对细胞极性建立的分子机制，为遗传性视网膜病变提供分子基础。
注：上述文献为示例，实际研究中建议通过PubMed/Google Scholar检索最新论文。
CCDC41 (Coiled-Coil Domain Containing Protein 41) is a conserved eukaryotic protein implicated in cellular processes related to cilia formation, cell cycle regulation, and intracellular transport. It is characterized by its coiled-coil domains, which facilitate protein-protein interactions and structural organization within cells. Studies suggest CCDC41 localizes to the centrosome and basal bodies, playing a role in microtubule anchoring and ciliogenesis. Its dysfunction has been linked to ciliopathies, a group of disorders affecting cilia-dependent functions such as vision, respiration, and embryonic development.
Recombinant CCDC41 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for mechanistic studies. Researchers employ it to investigate its structural properties, binding partners, and regulatory mechanisms in vitro. For example, recombinant CCDC41 has been used to demonstrate its interaction with key ciliary components like IFT88. supporting its role in intraflagellar transport. Additionally, it serves as an antigen for antibody development to study endogenous protein localization and expression patterns in disease models.
Current research focuses on elucidating how CCDC41 mutations contribute to pathological conditions. In cancer biology, altered CCDC41 expression correlates with tumor progression, potentially through dysregulated cell division. Its recombinant form also aids in high-throughput screening for therapeutic compounds targeting cilia-related pathways. Despite these advances, the full scope of CCDC41's biological functions and its tissue-specific roles remain under exploration, highlighting the importance of recombinant tools in advancing this field.
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