| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OARD1 |
| Uniprot No | Q9Y530 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-152aa |
| 氨基酸序列 | ASSLNEDPEGSRITYVKGDLFACPKTDSLAHCISEDCRMGAGIAVLFKKKFGGVQELLNQQKKSGEVAVLKRDGRYIYYLITKKRASHKPTYENLQKSLEAMKSHCLKNGVTDLSMPRIGCGLDRLQWENVSAMIEEVFEATDIKITVYTL |
| 预测分子量 | 24.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **OARD1重组蛋白** 的假设性参考文献示例(注：OARD1相关研究较少，以下内容基于文献结构推测，实际文献需通过数据库验证)：
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1. **标题**: *"OARD1 Recombinant Protein: Expression, Purification, and Antiviral Activity Analysis"*
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了OARD1重组蛋白的原核表达系统，通过亲和层析纯化获得高纯度蛋白。实验表明，OARD1重组蛋白能够显著抑制RNA病毒(如流感病毒)的复制，并激活下游干扰素信号通路，提示其在先天免疫中的潜在作用。
2. **标题**: *"Structural Characterization of OARD1 and Its Interaction with Viral RNA"*
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析了OARD1重组蛋白的三维结构，发现其具有典型的OAS家族结构域。体外结合实验证实，OARD1特异性识别病毒双链RNA，并可能通过构象变化触发宿主抗病毒反应。
3. **标题**: *"Functional Screening of OARD1 Variants in Autoimmune Diseases"*
**作者**: Tanaka K, et al.
**摘要**: 利用重组OARD1蛋白进行高通量筛选，发现某些单核苷酸多态性(SNPs)导致蛋白功能缺失，与系统性红斑狼疮(SLE)患者中异常免疫激活相关，为疾病机制研究提供了新方向。
4. **标题**: *"OARD1 Recombinant Protein as a Diagnostic Marker for Chronic Hepatitis B"*
**作者**: Wang Y, et al.
**摘要**: 研究开发了基于OARD1重组蛋白的ELISA检测方法，发现慢性乙肝患者血清中OARD1水平显著升高，提示其可作为肝纤维化进展的潜在生物标志物。
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**说明**：OARD1(2'-5' Oligoadenylate Synthetase-like Receptor Domain Containing 1)可能与抗病毒免疫或RNA识别相关，但实际文献需通过 **PubMed/Google Scholar** 以关键词“OARD1 recombinant protein”或“OARD1 protein function”检索核实。若研究较少，可扩展至其同源蛋白(如OAS家族)或相关通路文献。
OARD1 (Oxidation Resistance-Associated Domain-Containing Protein 1) is a conserved eukaryotic protein implicated in cellular stress responses and redox homeostasis. Initially identified through homology to bacterial oxidation resistance proteins, OARD1 contains a distinctive N-terminal domain (DUF1767) and a C-terminal OARD domain, both structurally linked to nucleic acid binding. Studies suggest its role in mitigating oxidative DNA damage by regulating replication stress and interacting with DNA repair machinery.
Recombinant OARD1 protein is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its purification often involves affinity tags like His-tags, enabling biochemical characterization of DNA/RNA binding properties and enzymatic activities. Emerging research highlights OARD1’s involvement in genome stability maintenance, with potential crosstalk between oxidative stress responses and innate immune signaling pathways. Dysregulation of OARD1 has been tentatively associated with cancer progression and neurodegenerative disorders, though mechanistic details remain under investigation.
As a research tool, recombinant OARD1 facilitates exploration of its structural motifs, post-translational modifications, and interactions with partners like PARP1 and R-loops. Its conserved nature across species makes it valuable for comparative studies in DNA damage response evolution. Current challenges include elucidating its precise molecular targets and developing assays to quantify its redox-modulatory functions in vitro.
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