| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STRAP |
| Uniprot No | Q9Y3F4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350aa |
| 氨基酸序列 | MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA |
| 预测分子量 | 65.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STRAP(丝氨酸/苏氨酸激酶受体相关蛋白)重组蛋白的模拟参考文献示例，供参考(注：部分信息可能为假设性概括，建议通过学术数据库核实最新文献)：
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1. **文献名称**：*STRAP regulates TGF-β signaling by stabilizing the TGF-β type I receptor kinase*
**作者**：Datta PK, et al.
**摘要**：研究揭示了STRAP通过结合并稳定TGF-β I型受体(TβRI)，增强其激酶活性，从而促进TGF-β/Smad信号通路的传导，并探讨其在肿瘤进展中的作用。
2. **文献名称**：*STRAP acts as a molecular chaperone to promote cancer metastasis via ERK signaling*
**作者**：Halder SK, et al.
**摘要**：报道了STRAP重组蛋白在胃癌细胞中的表达机制，证明其通过激活ERK信号通路增强肿瘤细胞侵袭性，并作为分子伴侣调控关键癌蛋白稳定性。
3. **文献名称**：*Interaction of STRAP with p53 modulates apoptosis in colorectal cancer*
**作者**：Pouladi N, et al.
**摘要**：通过体外重组蛋白实验，发现STRAP与p53直接结合并抑制其转录活性，导致结直肠癌细胞凋亡受阻，提示其作为潜在治疗靶点。
4. **文献名称**：*Recombinant STRAP protein expression and purification for functional studies*
**作者**：Chen L, et al.
**摘要**：描述了STRAP重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其与Smad蛋白的相互作用，为后续功能研究提供技术基础。
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**注意**：以上内容为示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如“STRAP recombinant protein”“STRAP cancer signaling”)获取。若需具体文献，请提供更多研究背景或访问学术数据库。
**Background of STRAP Recombinant Protein**
STRAP (Serine-Threonine Kinase Receptor-Associated Protein) is a multifunctional adaptor protein implicated in regulating diverse cellular processes, including signal transduction, cell proliferation, and apoptosis. It belongs to the WD40-repeat protein family, characterized by its conserved β-propeller structure formed by WD40 repeats, which mediate protein-protein interactions. STRAP acts as a scaffold, coordinating interactions between kinases, transcription factors, and other signaling molecules.
A key role of STRAP is its involvement in the TGF-β (Transforming Growth Factor-Beta) signaling pathway, where it modulates SMAD protein activity. By binding to SMAD7 and type I TGF-β receptors, STRAP influences SMAD complex formation, thereby regulating TGF-β-mediated responses such as cell differentiation, fibrosis, and immune regulation. Additionally, STRAP interacts with stress-activated kinases (e.g., p38 MAPK) and PI3K/AKT pathways, linking it to cellular stress responses and survival mechanisms.
In cancer biology, STRAP exhibits dual roles. It can act as an oncogene by promoting tumor cell survival, migration, and chemoresistance in cancers like glioblastoma or colorectal carcinoma. Conversely, it may function as a tumor suppressor in certain contexts by stabilizing p53 or inhibiting EMT (Epithelial-Mesenchymal Transition). Dysregulation of STRAP expression is associated with poor prognosis in multiple malignancies, highlighting its therapeutic potential.
Recombinant STRAP proteins are engineered to study these mechanisms. Produced via bacterial or mammalian expression systems, they retain functional domains for in vitro binding assays, structural studies, or screening for inhibitors. Tagged versions (e.g., His-, GST-, or FLAG-tagged) facilitate purification and detection. Such tools are vital for deciphering STRAP’s post-translational modifications (e.g., phosphorylation, ubiquitination) and its crosstalk with signaling networks, aiding drug development for cancers and fibrotic diseases.
In summary, STRAP’s versatility in cellular signaling and disease contexts makes it a compelling target for biomedical research, with recombinant proteins serving as essential reagents for mechanistic and translational studies.
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