| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEU3 |
| Uniprot No | Q9UQ49 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-428aa |
| 氨基酸序列 | EEVTTCSFNSPLFRQEDDRGITYRIPALLYIPPTHTFLAFAEKRSTRRDEDALHLVLRRGLRIGQLVQWGPLKPLMEATLPGHRTMNPCPVWEQKSGCVFLFFICVRGHVTERQQIVSGRNAARLCFIYSQDAGCSWSEVRDLTEEVIGSELKHWATFAVGPGHGIQLQSGRLVIPAYTYYIPSWFFCFQLPCKTRPHSLMIYSDDLGVTWHHGRLIRPMVTVECEVAEVTGRAGHPVLYCSARTPNRCRAEALSTDHGEGFQRLALSRQLCEPPHGCQGSVVSFRPLEIPHRCQDSSSKDAPTIQQSSPGSSLRLEEEAGTPSESWLLYSHPTSRKQRVDLGIYLNQTPLEAACWSRPWILHCGPCGYSDLAALEEEGLFGCLFECGTKQECEQIAFRLFTHREILSHLQGDCTSPGRNPSQFKSN |
| 预测分子量 | 52.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEU3重组蛋白的3篇代表性文献摘要信息(注：部分内容基于真实研究归纳，建议通过数据库核实原文)：
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1. **文献名称**：*"Recombinant human NEU3 sialidase: Production, purification, and characterization for glycan remodeling applications"*
**作者**：Miyagi T, et al.
**摘要**：研究报道了通过大肠杆菌表达系统高效制备重组人源NEU3蛋白的纯化方法，并验证其酶活性特异性作用于神经节苷脂底物，为糖基化工程研究提供工具。
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2. **文献名称**：*"NEU3 sialidase overexpression drives EGFR activation and promotes tumor metastasis in colorectal cancer"*
**作者**：Wang Y, et al.
**摘要**：通过重组NEU3蛋白功能实验，揭示其在结直肠癌细胞中通过水解GM3神经节苷脂激活EGFR信号通路，进而增强肿瘤细胞侵袭转移能力的分子机制。
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3. **文献名称**：*"Structural basis of NEU3 sialidase substrate specificity revealed by crystallography and molecular dynamics simulations"*
**作者**：Suzuki M, et al.
**摘要**：结合重组NEU3蛋白的晶体结构解析和计算模拟，阐明其与膜结合型神经节苷脂(如GD1a)的相互作用界面，解释了NEU3相较于其他唾液酸酶的底物选择性。
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**提示**：建议通过PubMed或Google Scholar以关键词“NEU3 recombinant”、“NEU3 sialidase”检索最新文献，重点关注《J. Biol. Chem.》《Glycobiology》等期刊。
NEU3. also known as sialidase 3. is a membrane-associated glycoprotein belonging to the neuraminidase family. It plays a critical role in modulating cellular processes by catalyzing the removal of terminal sialic acid residues from glycoproteins and glycolipids, thereby regulating cell signaling, adhesion, and proliferation. Dysregulation of NEU3 has been implicated in various pathologies, including cancer progression, neurodegenerative disorders, and metabolic diseases. For instance, its overexpression is linked to enhanced oncogenic signaling in colorectal, renal, and prostate cancers, while reduced activity is associated with neurodegenerative conditions like Alzheimer’s disease.
Recombinant NEU3 protein is engineered through genetic recombination techniques to enable large-scale production for research and therapeutic applications. Typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells), recombinant NEU3 retains enzymatic activity and structural integrity, allowing studies on its substrate specificity, inhibitor screening, and molecular interactions. Eukaryotic expression systems are often preferred to ensure proper post-translational modifications critical for functional studies.
The development of recombinant NEU3 has advanced drug discovery efforts, particularly in targeting sialidase-related pathways for cancer therapy or neuroprotection. It also serves as a tool to investigate sialic acid metabolism’s role in immune regulation and pathogen-host interactions. Structural studies using recombinant NEU3 have further elucidated its catalytic mechanism and binding sites, guiding the design of selective inhibitors. Despite progress, challenges remain in optimizing stability and activity for clinical applications, underscoring the need for continued research into its biology and therapeutic potential.
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