| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | pos5 |
| Uniprot No | Q06892 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-414aa |
| 氨基酸序列 | MFVRVKLNKPVKWYRFYSTLDSHSLKLQSGSKFVKIKPVNNLRSSSSADFVSPPNSKLQSLIWQNPLQNVYITKKPWTPSTREAMVEFITHLHESYPEVNVIVQPDVAEEISQDFKSPLENDPNRPHILYTGPEQDIVNRTDLLVTLGGDGTILHGVSMFGNTQVPPVLAFALGTLGFLSPFDFKEHKKVFQEVISSRAKCLHRTRLECHLKKKDSNSSIVTHAMNDIFLHRGNSPHLTNLDIFIDGEFLTRTTADGVALATPTGSTAYSLSAGGSIVSPLVPAILMTPICPRSLSFRPLILPHSSHIRIKIGSKLNQKPVNSVVKLSVDGIPQQDLDVGDEIYVINEVGTIYIDGTQLPTTRKTENDFNNSKKPKRSGIYCVAKTENDWIRGINELLGFNSSFRLTKRQTDND |
| 预测分子量 | 46,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于POS5重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *Functional characterization of yeast POS5 recombinant protein in mitochondrial NADPH homeostasis*
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌中表达并纯化了酿酒酵母POS5重组蛋白，证实其作为线粒体NAD激酶的活性，揭示了POS5通过生成NADPH维持氧化还原平衡的分子机制。
2. **文献名称**: *Crystal structure analysis of recombinant POS5 from Saccharomyces cerevisiae*
**作者**: Lee B, et al.
**摘要**: 通过X射线晶体学解析了重组ScPOS5蛋白的三维结构，发现其ATP结合域的关键氨基酸残基，为设计靶向该酶的抑制剂提供结构基础。
3. **文献名称**: *POS5 recombinant protein enhances stress tolerance in industrial yeast strains*
**作者**: Chen L, et al.
**摘要**: 将重组POS5蛋白导入工业酵母菌株，实验显示其过表达显著提升细胞对氧化应激和高温的耐受性，证明其在合成生物学中的应用潜力。
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**注意**：以上文献为模拟生成，实际文献需通过PubMed、Web of Science等学术平台检索。建议结合关键词 "POS5 gene"、"recombinant NAD kinase"、"Saccharomyces cerevisiae mitochondrial" 进行精准查询。
**Background of POS5 Recombinant Protein**
POS5. a mitochondrial NADH kinase in *Saccharomyces cerevisiae*, plays a critical role in cellular redox balance by catalyzing the phosphorylation of NADH to generate NADPH. This enzyme is essential for maintaining mitochondrial function, particularly under oxidative stress, as NADPH serves as a cofactor for antioxidant systems like the thioredoxin and glutathione pathways. POS5-deficient yeast strains exhibit hypersensitivity to oxidative agents, underscoring its importance in stress response and mitochondrial homeostasis.
The recombinant POS5 protein is engineered through heterologous expression systems (e.g., *E. coli* or yeast) to study its structural and functional properties. Cloning the *POS5* gene into expression vectors allows large-scale production of the purified protein, enabling biochemical assays to characterize its enzymatic activity, substrate specificity, and interaction partners. Recombinant POS5 has been instrumental in elucidating mechanisms of NADPH regeneration, mitochondrial metabolism, and oxidative damage repair.
Research on POS5 also extends to biomedical and biotechnological applications. Its role in redox regulation links it to aging, neurodegenerative diseases, and cancer, where mitochondrial dysfunction is a hallmark. In industrial biotechnology, engineering POS5 activity could enhance microbial stress tolerance in biofuel production or chemical synthesis.
Despite its significance, POS5's regulatory mechanisms and post-translational modifications remain under investigation. Structural studies using recombinant protein aim to resolve its 3D conformation, aiding in drug discovery for diseases linked to mitochondrial NADPH dysregulation. Overall, POS5 recombinant protein serves as a vital tool for advancing both basic science and applied research in redox biology and mitochondrial medicine.
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