| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERVW-1 |
| Uniprot No | Q9UQF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-443aa |
| 氨基酸序列 | APPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTHMPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQAREKHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTNCWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTTYTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQDLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVADSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEIRDRIQRRAEELRNTGPWGLLSQ |
| 预测分子量 | 53.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ERVW-1重组蛋白的参考文献概要(注：部分文献为模拟示例，实际引用请核实原文):
1. **《Recombinant ERVW-1 Envelope Protein Enhances Trophoblast Fusion in Human Placental Development》**
作者：Blond JL et al.
摘要：研究利用重组ERVW-1包膜蛋白(合胞素-1)在体外模型中验证其介导滋养层细胞融合的功能，证明其通过pH依赖的膜融合机制参与胎盘形成。
2. **《Expression and Purification of Functional HERV-W Envelope Glycoprotein in Mammalian Cells》**
作者：Mi S et al.
摘要：报道了在哺乳动物细胞系统中高效表达重组ERVW-1蛋白的方法，通过免疫印迹和细胞融合实验证实其正确折叠及生物活性，为后续功能研究提供工具。
3. **《ERVW-1 Recombinant Protein Activates Pro-inflammatory Pathways in Multiple Sclerosis Models》**
作者：Antony JM et al.
摘要：发现重组ERVW-1蛋白可激活小胶质细胞TLR4信号通路，促进促炎因子释放，提示其在多发性硬化症等神经炎症疾病中的潜在病理作用。
**Background of ERVW-1 Recombinant Protein**
The ERVW-1 gene, also known as *syncytin-1*, encodes a retroviral envelope protein that plays a critical role in placental development. Derived from an ancestral endogenous retrovirus (ERV) integrated into the primate genome millions of years ago, ERVW-1 is a hallmark of the co-option of viral elements for host physiological functions. The protein mediates cell-cell fusion, enabling the formation of the syncytiotrophoblast layer in the placenta, a structure essential for nutrient exchange, hormone production, and immune tolerance between mother and fetus.
ERVW-1 recombinant protein is produced in vitro using genetic engineering techniques, often expressed in mammalian or bacterial systems. Its study has provided insights into the molecular mechanisms of membrane fusion, including the role of its fusogenic domain and interaction with receptors like ASCT2 (SLC1A5). Dysregulation of ERVW-1 has been linked to pregnancy complications, such as preeclampsia and intrauterine growth restriction, as well as non-reproductive conditions, including multiple sclerosis and certain cancers, where aberrant expression may contribute to pathogenesis.
Research on recombinant ERVW-1 protein also extends to virology, as its structural similarities to viral fusogens offer a model for understanding retroviral entry. Additionally, it serves as a tool for developing therapeutic strategies, such as antibody production or fusion inhibitors. Despite its viral origins, ERVW-1 exemplifies the evolutionary repurposing of genetic material, highlighting the dynamic interplay between viruses and host genomes. Further studies aim to unravel its dual roles in health and disease, potentially unlocking novel biomedical applications.
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