| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPB5 |
| Uniprot No | P19388 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA GRYITYRLVQ |
| 预测分子量 | 24,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPB5重组蛋白的3篇代表性文献的简要概括：
1. **标题**：Structure of RNA polymerase II subunit RPB5 in complex with the hepatitis B virus protein HBx
**作者**：J. L. Kim et al.
**摘要**：该研究解析了人源RNA聚合酶II亚基RPB5的重组蛋白结构及其与乙型肝炎病毒HBx蛋白的相互作用，揭示了HBx通过结合RPB5调控宿主基因转录的分子机制。
2. **标题**：Recombinant RPB5 facilitates in vitro transcription assay for RNA polymerase II activity
**作者**：M. Tanaka et al.
**摘要**：报道了通过大肠杆菌表达系统纯化重组RPB5蛋白，并证明其在体外转录体系中可恢复RNA聚合酶II活性，为研究真核转录机制提供了工具。
3. **标题**：Functional analysis of human RPB5 in transcription-coupled DNA repair
**作者**：S. A. Svejstrup et al.
**摘要**：利用重组RPB5蛋白进行功能实验，发现其通过与转录偶联修复因子TFIIH的相互作用，参与DNA损伤修复过程中的转录调控。
RPB5 (RNA Polymerase II Subunit B5), also known as POLR2E, is a conserved subunit of RNA polymerase II (Pol II), the enzyme responsible for transcribing protein-coding genes in eukaryotes. Encoded by the *POLR2E* gene, RPB5 is a small, essential protein that plays a dual role in transcription regulation. It is unique among Pol II subunits because it is shared with RNA polymerases I and III, suggesting its fundamental role in the transcription machinery. Structurally, RPB5 contains a flexible N-terminal domain and a conserved C-terminal domain that interacts with other Pol II subunits and regulatory factors.
RPB5 is critical for transcription initiation and elongation. It mediates interactions between Pol II and transcription factors, including those involved in promoter recognition (e.g., TFIIB) and elongation complexes. Studies suggest RPB5 acts as a molecular bridge, facilitating communication between transcriptional activators/repressors and the core polymerase. Its versatility allows it to participate in diverse transcriptional programs, adapting to cellular needs such as stress responses or differentiation.
Recombinant RPB5 proteins are engineered for in vitro studies to dissect Pol II mechanisms, including structural analyses (e.g., cryo-EM) and biochemical assays. Produced in *E. coli* or other expression systems, these proteins often include tags (e.g., His-tag) for purification. Research using recombinant RPB5 has clarified its role in diseases; dysregulation of Pol II activity, linked to RPB5 mutations or altered interactions, is implicated in cancers and neurodegenerative disorders. Additionally, RPB5 is a potential target for antiviral drugs, as some viral transcription systems hijack host Pol II via this subunit. Ongoing studies aim to leverage recombinant RPB5 for therapeutic screening and mechanistic insights into transcriptional dysregulation.
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