| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNRD2 |
| Uniprot No | Q9NNW7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-524aa |
| 氨基酸序列 | QRDYDLLVVGGGSGGLACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCSG |
| 预测分子量 | 68.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TXNRD2重组蛋白的3篇代表性文献(示例为模拟构造，仅供参考)：
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1. **标题**: *"Recombinant human TXNRD2: production, purification, and enzymatic characterization"*
**作者**: Müller S, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人TXNRD2蛋白，通过体外酶活实验证实其具有硫氧还蛋白依赖性还原酶活性，为后续功能研究提供了可靠工具。
2. **标题**: *"TXNRD2 overexpression enhances chemoresistance in colorectal cancer via mitochondrial antioxidant pathways"*
**作者**: Zhang L, Wang Y, et al.
**摘要**: 文章通过构建TXNRD2重组蛋白过表达的结直肠癌细胞模型，发现其通过激活线粒体抗氧化通路降低ROS水平，进而增强癌细胞对5-氟尿嘧啶的耐药性。
3. **标题**: *"Structural insights into TXNRD2 mechanism and its inhibition by auranofin"*
**作者**: Cenas N, et al.
**摘要**: 利用X射线晶体学解析了重组TXNRD2的三维结构，并揭示抗风湿药物金诺芬通过共价结合其活性中心硒代半胱氨酸残基抑制酶活性的分子机制。
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*注：以上文献信息为示例性构造，实际引用需以真实出版物为准。建议通过PubMed或Web of Science以关键词“TXNRD2 recombinant”或“thioredoxin reductase 2”检索最新研究。*
**Background of TXNRD2 Recombinant Protein**
Thioredoxin reductase 2 (TXNRD2) is a mitochondrial isoform of the thioredoxin reductase family, playing a critical role in maintaining cellular redox homeostasis. As a selenocysteine-containing flavoprotein, TXNRD2 catalyzes the reduction of thioredoxin 2 (Trx2) using NADPH as an electron donor, thereby supporting antioxidant defense systems, regulating mitochondrial oxidative stress, and modulating redox-sensitive signaling pathways. This enzyme is essential for protecting mitochondrial DNA, proteins, and lipids from oxidative damage, and it participates in processes such as apoptosis, cell proliferation, and energy metabolism.
TXNRD2 dysfunction has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and cancer. Its activity is tightly linked to mitochondrial integrity, making it a potential therapeutic target for conditions associated with oxidative stress or mitochondrial impairment. For instance, overexpression of TXNRD2 has been observed in certain tumors, suggesting a role in cancer progression and resistance to chemotherapy.
Recombinant TXNRD2 protein is produced via genetic engineering techniques, often expressed in bacterial or eukaryotic systems with affinity tags (e.g., His-tag) for purification. This engineered protein retains enzymatic activity and is widely used in biochemical assays, structural studies, and drug discovery to investigate TXNRD2's mechanisms, screen inhibitors, or explore its interactions with cellular components. Researchers also employ recombinant TXNRD2 to model mitochondrial redox dysregulation in vitro, aiding the development of targeted therapies.
In summary, TXNRD2 recombinant protein serves as a vital tool for dissecting mitochondrial redox biology and advancing therapeutic strategies against oxidative stress-related diseases.
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