| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLG2 |
| Uniprot No | Q9UHN1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485aa |
| 氨基酸序列 | MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV |
| 预测分子量 | 70.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLG2重组蛋白的3篇代表性文献摘要：
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1. **标题**：Expression and functional characterization of the human mitochondrial DNA polymerase gamma holoenzyme
**作者**：Johnson AA, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人POLG2蛋白，证实其作为POLG1的辅助亚基，显著增强全酶的DNA结合能力和持续合成能力，揭示了二者协同作用机制。
2. **标题**：Structural basis for processivity and toxicity of human mitochondrial DNA polymerase gamma
**作者**：Lee YS, et al.
**摘要**：通过冷冻电镜解析POLG1-POLG2重组复合体的三维结构，发现POLG2通过特定结构域稳定POLG1的构象，为某些神经退行性疾病相关突变提供分子解释。
3. **标题**：Recombinant POLG2 variants impair mitochondrial DNA replication in dominant ataxia patients
**作者**：Smith RL, et al.
**摘要**：利用重组技术构建POLG2致病突变体，发现其异常结合POLG1导致DNA复制效率下降，首次将POLG2显性突变与小脑共济失调直接关联。
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注：以上文献为虚拟概括，实际研究需通过PubMed/Google Scholar以关键词“POLG2 recombinant”“mitochondrial DNA polymerase gamma”检索具体论文。
**Background of POLG2 Recombinant Protein**
POLG2. also known as the mitochondrial DNA polymerase γ subunit B, is a critical regulatory component of the mitochondrial DNA (mtDNA) replication machinery. It functions as the accessory subunit of the heterodimeric DNA polymerase γ (Pol γ), which is essential for replicating and repairing mtDNA. The catalytic subunit, POLG1. carries the polymerase and exonuclease activities, while POLG2 enhances processivity, stabilizes the holoenzyme, and modulates its enzymatic fidelity. Together, they ensure the high-fidelity replication of mtDNA, maintaining mitochondrial genome integrity and cellular energy production.
Dysregulation of POLG2 is linked to mitochondrial disorders, neurodegenerative diseases, and aging. Mutations in POLG2. though less common than those in POLG1. can disrupt mtDNA replication, leading to mtDNA depletion or deletions. These defects contribute to tissue-specific pathologies, particularly in energy-demanding organs like the brain, heart, and muscles. Studying POLG2’s molecular mechanisms is thus vital for understanding mitochondrial dysfunction and associated diseases.
Recombinant POLG2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling in vitro exploration of its structure, interactions, and functional roles. Its recombinant form is used to reconstitute Pol γ activity in biochemical assays, screen therapeutic compounds targeting mitochondrial replication, and model disease-associated mutations. Additionally, it supports gene therapy research aimed at restoring mtDNA replication in POLG-related disorders.
Recent advances in structural biology, such as cryo-EM, have elucidated POLG2’s role in stabilizing POLG1 and coordinating with other replication factors. These insights underscore its potential as a therapeutic target for mitochondrial diseases, cancer (where mtDNA instability is common), and age-related metabolic decline. Overall, POLG2 recombinant protein serves as a key tool in mitochondrial research, bridging molecular insights to clinical applications.
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