| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASH2L |
| Uniprot No | Q9UBL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534aa |
| 氨基酸序列 | MDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSGKGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP |
| 预测分子量 | 76.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASH2L重组蛋白的3篇代表性文献，包含标题、作者及摘要概括：
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1. **标题**: *Structural basis for activity regulation of MLL family methyltransferases*
**作者**: Chen S, et al.
**摘要**: 本研究解析了包含ASH2L重组蛋白的MLL复合体晶体结构，揭示了ASH2L与WDR5、RbBP5等蛋白的相互作用机制，阐明其在调控组蛋白H3K4甲基转移酶活性中的关键作用。
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2. **标题**: *Reconstitution and functional analysis of the human SET1/MLL histone H3K4 methyltransferase complex*
**作者**: Southall SM, et al.
**摘要**: 通过重组表达ASH2L、WDR5、RbBP5等蛋白，体外重建了功能性SET1/MLL复合体，证明ASH2L是复合体催化H3K4甲基化不可或缺的组分，并影响其底物特异性。
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3. **标题**: *ASH2L regulates TLR4 signaling pathway via histone methylation in macrophages*
**作者**: Li Y, et al.
**摘要**: 利用重组ASH2L蛋白进行体外互作实验，发现其通过调控促炎基因的H3K4me3修饰影响巨噬细胞TLR4信号通路，为ASH2L在免疫反应中的表观遗传功能提供证据。
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如需具体文献DOI或补充更多研究，可进一步说明。
ASH2L (Absent, Small, or Homeotic Discs 2-Like) is a conserved nuclear protein that plays a critical role in epigenetic regulation as a core component of the COMPASS (Complex of Proteins Associated with Set1) family of histone methyltransferase complexes. These complexes catalyze the trimethylation of histone H3 at lysine 4 (H3K4me3), a chromatin modification strongly associated with active gene promoters. ASH2L stabilizes the interaction between COMPASS subunits and enhances the methyltransferase activity of the catalytic SET domain proteins, such as MLL1/2 in mammals. Its function is essential for embryonic development, stem cell maintenance, and cellular differentiation.
Recombinant ASH2L protein is produced using heterologous expression systems (e.g., E. coli, insect cells) to enable biochemical and structural studies. Purified ASH2L retains the ability to bind other COMPASS components like WDR5. RbBP5. and DPY30. forming a minimal subcomplex required for optimal H3K4 methylation. Researchers utilize recombinant ASH2L to investigate molecular mechanisms of histone modification, including how mutations or dysregulation contribute to diseases like cancers, developmental disorders, and leukemia. For example, aberrant ASH2L expression correlates with poor prognosis in multiple malignancies, making it a potential therapeutic target. Additionally, recombinant ASH2L serves as a tool for screening small-molecule inhibitors targeting COMPASS activity. Recent structural studies using recombinant protein have revealed its winged-helix domain architecture and dynamic interactions within the methyltransferase complex, advancing our understanding of epigenetic regulation at atomic resolution. Its role in maintaining pluripotency and cell fate decisions also positions it as a focus in regenerative medicine research.
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