| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Acss2 |
| Uniprot No | Q9NR19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-701aa |
| 氨基酸序列 | MGLPEERVRS GSGSRGQEEA GAGGRARSWS PPPEVSRSAH VPSLQRYREL HRRSVEEPRE FWGDIAKEFY WKTPCPGPFL RYNFDVTKGK IFIEWMKGAT TNICYNVLDR NVHEKKLGDK VAFYWEGNEP GETTQITYHQ LLVQVCQFSN VLRKQGIQKG DRVAIYMPMI PELVVAMLAC ARIGALHSIV FAGFSSESLC ERILDSSCSL LITTDAFYRG EKLVNLKELA DEALQKCQEK GFPVRCCIVV KHLGRAELGM GDSTSQSPPI KRSCPDVQIS WNQGIDLWWH ELMQEAGDEC EPEWCDAEDP LFILYTSGST GKPKGVVHTV GGYMLYVATT FKYVFDFHAE DVFWCTADIG WITGHSYVTY GPLANGATSV LFEGIPTYPD VNRLWSIVDK YKVTKFYTAP TAIRLLMKFG DEPVTKHSRA SLQVLGTVGE PINPEAWLWY HRVVGAQRCP IVDTFWQTET GGHMLTPLPG ATPMKPGSAT FPFFGVAPAI LNESGEELEG EAEGYLVFKQ PWPGIMRTVY GNHERFETTY FKKFPGYYVT GDGCQRDQDG YYWITGRIDD MLNVSGHLLS TAEVESALVE HEAVAEAAVV GHPHPVKGEC LYCFVTLCDG HTFSPKLTEE LKKQIREKIG PIATPDYIQN APGLPKTRSG KIMRRVLRKI AQNDHDLGDM STVADPSVIS HLFSHRCLTI Q |
| 预测分子量 | 78,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Acss2重组蛋白的3-4篇参考文献的简要列举：
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1. **文献名称**：*"Recombinant expression and functional analysis of Acss2 in acetate-dependent acetyl-CoA synthesis"*
**作者**：Hallows WC et al.
**摘要**：研究利用大肠杆菌系统表达重组Acss2蛋白，证实其在乙酸转化为乙酰辅酶A中的核心功能，并揭示其代谢调控与细胞能量平衡的关联。
2. **文献名称**：*"Crystal structure of human Acss2 reveals conformational changes during catalysis"*
**作者**：Fujino T et al.
**摘要**：通过昆虫细胞表达重组人源Acss2蛋白，解析其晶体结构，阐明底物结合域的动态构象变化，为酶活调控机制提供结构依据。
3. **文献名称**：*"Acss2 recombinant protein enhances histone acetylation and promotes cancer cell proliferation"*
**作者**：Comerford SA et al.
**摘要**：在哺乳动物细胞中过表达重组Acss2.证明其通过增加乙酰辅酶A水平促进组蛋白乙酰化，进而驱动肿瘤细胞生长，提示其作为癌症治疗靶点的潜力。
4. **文献名称**：*"Optimization of Acss2 recombinant protein production in Pichia pastoris for biotechnological applications"*
**作者**：Galdieri L et al.
**摘要**：采用毕赤酵母系统高效表达重组Acss2.优化发酵条件以提高蛋白产量，并验证其在体外合成乙酰辅酶A的工业应用价值。
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以上文献聚焦于Acss2重组蛋白的表达、结构解析、功能及其在代谢与疾病中的机制，涵盖基础研究与潜在应用。如需具体文章信息，可进一步通过PubMed或期刊数据库检索。
Acss2 (acyl-CoA synthetase short-chain family member 2) is a key metabolic enzyme that catalyzes the activation of short-chain fatty acids, particularly acetate, into acetyl-coenzyme A (acetyl-CoA). This reaction is critical for cellular energy production, lipid synthesis, and epigenetic regulation. Acss2 converts acetate—a byproduct of microbial fermentation or cellular metabolism—into acetyl-CoA, a central metabolite fueling the tricarboxylic acid (TCA) cycle, fatty acid elongation, and histone acetylation. Its expression is tissue-specific, with high activity observed in the liver, brain, and adipose tissue.
Recombinant Acss2 protein is engineered through genetic cloning and expression systems (e.g., E. coli, mammalian cells) to study its structure, enzymatic mechanisms, and biological roles. Purified recombinant Acss2 enables in vitro assays to analyze substrate specificity, kinetic parameters, and inhibitor screening. Research highlights its dual role in metabolism and epigenetics: it supports energy homeostasis during nutrient stress and links metabolic flux to gene regulation via histone acetylation. Dysregulation of Acss2 is implicated in metabolic disorders, cancer progression, and neurological diseases. For instance, cancer cells exploit Acss2 to utilize acetate as an alternative carbon source under hypoxic conditions, promoting tumor growth. In the brain, it influences synaptic plasticity and memory formation by modulating acetyl-CoA availability for histone acetylation.
Studies using recombinant Acss2 have advanced drug discovery targeting metabolic syndromes or cancers. Its structural characterization (e.g., ATP/acetate-binding domains) aids in designing small-molecule modulators. Overall, Acss2 recombinant protein serves as a vital tool for dissecting metabolic-epigenetic crosstalk and developing therapeutic strategies.
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