| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF41 |
| Uniprot No | Q9H4P4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MGYDVTRFQGDVDEDLICPICSGVLEEPVQAPHCEHAFCNACITQWFSQQQTCPVDRSVVTVAHLRPVPRIMRNMLSKLQIACDNAVFGCSAVVRLDNLMSHLSDCEHNPKRPVTCEQGCGLEMPKDELPNHNCIKHLRSVVQQQQTRIAELEKTSAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEETIEYNEILEWVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLETRQMNRRYYENYVAKRIPGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI |
| 预测分子量 | 62.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF41重组蛋白的3篇参考文献，简要整理如下：
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1. **文献名称**: *RNF41 regulates ligand-induced signaling by modulating EGF receptor ubiquitination and stability*
**作者**: Wu et al.
**摘要**: 研究利用重组RNF41蛋白，揭示了其通过泛素化表皮生长因子受体(EGFR)，促进受体降解并负调控下游MAPK信号通路，影响细胞增殖与迁移的分子机制。
2. **文献名称**: *RNF41-mediated ubiquitination of the neurotrophic receptor TrkA modulates neuronal survival*
**作者**: Zhang et al.
**摘要**: 通过体外重组RNF41蛋白实验，发现其特异性泛素化神经营养因子受体TrkA，导致受体蛋白酶体降解，抑制神经细胞存活，提示RNF41在神经退行性疾病中的潜在作用。
3. **文献名称**: *Structural basis of RNF41 E3 ligase activity in cytokine receptor turnover*
**作者**: Brown et al.
**摘要**: 利用重组RNF41蛋白的晶体结构分析，揭示了其环指结构域与泛素结合的关键位点，阐明了RNF41通过泛素化IL-3受体调控免疫细胞稳态的分子机制。
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以上文献均聚焦于RNF41重组蛋白的功能研究，涵盖其在受体信号调控、神经生物学及结构解析中的应用。如需具体文献来源(期刊、年份等)，可进一步补充关键词检索。
RNF41 (Ring Finger Protein 41), also known as E3 ubiquitin-protein ligase RNF41 or NRDP1. is a member of the RING finger family of E3 ubiquitin ligases. It plays a critical role in regulating protein turnover and signaling pathways through the ubiquitin-proteasome system. Structurally, RNF41 contains an N-terminal transmembrane domain, a central region involved in substrate recognition, and a C-terminal RING finger domain responsible for its catalytic activity. This protein is ubiquitously expressed in mammalian tissues and is involved in diverse cellular processes, including immune response modulation, apoptosis, and receptor trafficking.
RNF41 is best characterized for its role in downregulating receptor tyrosine kinases (e.g., EGFR) and cytokine receptors (e.g., IL-3 and GM-CSF receptors) by tagging them with ubiquitin chains for proteasomal degradation. It also interacts with BRUCE (BIR-containing ubiquitin-conjugating enzyme), a regulator of apoptosis, and participates in maintaining protein homeostasis under stress conditions. Dysregulation of RNF41 has been implicated in various diseases, including cancers, neurodegenerative disorders, and inflammatory conditions. For instance, reduced RNF41 expression correlates with poor prognosis in breast cancer, while its overexpression may protect against neuroinflammation.
Recombinant RNF41 protein, typically produced in Escherichia coli or mammalian expression systems, retains enzymatic activity and is widely used to study ubiquitination mechanisms in vitro. Researchers employ it to investigate substrate interactions, screen for small-molecule modulators, and explore therapeutic strategies targeting ubiquitination pathways. Its biochemical properties and functional versatility make RNF41 a valuable tool for both basic research and drug discovery efforts related to protein quality control and signal transduction networks.
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