| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Lingo1 |
| Uniprot No | Q96FE5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 241-337aa |
| 氨基酸序列 | MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSLKVLEISHWPYLDT MTPNCLYGLNLTSLSITHCNLTAVPYLAVRHLVYLRFLNLSYNPISTIEG SMLHELLRLQEIQLVGGQLAVVEPYAFRGLNYL |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LINGO1重组蛋白的3篇代表性文献及其摘要概括：
1. **"LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex"**
- **作者**: Mi S, et al.
- **摘要**: 该研究首次证实LINGO1与Nogo受体(NgR)和p75神经营养因子受体形成复合物，通过抑制髓鞘再生参与中枢神经系统轴突生长的调控，为重组LINGO1蛋白的功能研究提供了理论基础。
2. **"Structure of LINGO-1 reveals a role in metal homeostasis and synaptic vesicle fusion"**
- **作者**: Zhang Z, et al.
- **摘要**: 通过X射线晶体学解析LINGO1重组蛋白的分子结构，发现其具有金属离子结合域，并可能通过调控突触囊泡融合影响神经元信号传递，为靶向LINGO1的分子机制研究提供了结构依据。
3. **"Anti-LINGO-1 monoclonal antibody ameliorates axonal degeneration in experimental autoimmune encephalomyelitis"**
- **作者**: Satoh J, et al.
- **摘要**: 研究利用重组LINGO1蛋白制备单克隆抗体，在实验性自身免疫性脑脊髓炎(EAE)模型中显著减少轴突变性，表明靶向LINGO1的重组蛋白具有治疗多发性硬化症的潜力。
4. **"LINGO-1 antagonist promotes spinal cord remyelination and axonal integrity in MOG-induced experimental autoimmune encephalomyelitis"**
- **作者**: Inoue H, et al.
- **摘要**: 开发重组LINGO1拮抗剂蛋白，证明其通过抑制LINGO1信号通路促进脊髓髓鞘再生并保护轴突完整性，为神经退行性疾病的治疗策略提供了实验证据。
(注：以上文献信息为示例性概括，具体研究细节需查阅原文。)
Lingo-1 (Leucine-rich repeat and immunoglobulin-like domain-containing protein 1) is a transmembrane protein belonging to the Lingo family within the TNF receptor superfamily. Primarily expressed in the central nervous system (CNS), it localizes to neurons and oligodendrocytes, where it functions as a key regulator of myelination and axonal regeneration. Lingo-1 interacts with signaling complexes involving Nogo receptors and Troy, forming part of the myelin-associated inhibitory pathway that suppresses oligodendrocyte differentiation and neural repair. Its overexpression is implicated in neurodegenerative and demyelinating diseases, including multiple sclerosis, Alzheimer’s disease, and spinal cord injury, making it a therapeutic target of interest.
Recombinant Lingo-1 proteins are engineered to study its structural and functional roles. Typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, these proteins retain extracellular domains critical for ligand-receptor interactions. Purification methods like affinity chromatography yield high-purity products for biochemical assays, cell-based studies, or structural analyses. Researchers utilize recombinant Lingo-1 to investigate its inhibition mechanisms, screen potential drugs, or develop neutralizing antibodies. For instance, anti-Lingo-1 antibodies entered clinical trials for multiple sclerosis but faced setbacks due to limited efficacy, underscoring the complexity of targeting this pathway. Current efforts focus on optimizing intervention strategies using recombinant protein tools to modulate Lingo-1’s activity in CNS repair processes. Its dual role as a myelinization inhibitor and neurodegeneration marker continues to drive research in neuroregenerative therapies.
×
