| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL11A |
| Uniprot No | Q9BV86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEFMTSEV IEDEKQFYSK AKTYWKQIPP TVDGMLGGYG HISSIDINSS RKFLQRFLRE GPNKTGTSCA LDCGAGIGRI TKRLLLPLFR EVDMVDITED FLVQAKTYLG EEGKRVRNYF CCGLQDFTPE PDSYDVIWIQ WVIGHLTDQH LAEFLRRCKG SLRPNGIIVI KDNMAQEGVI LDDVDSSVCR DLDVVRRIIC SAGLSLLAEE RQENLPDEIY HVYSFALR |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于METTL11A重组蛋白的3篇参考文献示例(注：文献为模拟示例，实际引用时需核实真实来源)：
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1. **文献名称**：*"Recombinant METTL11A Methyltransferase: Biochemical Characterization and Substrate Specificity"*
**作者**：Wang et al.
**摘要**：研究通过大肠杆菌系统表达并纯化METTL11A重组蛋白，证实其作为组蛋白H3K36特异性甲基转移酶的活性，揭示了其对S-腺苷甲硫氨酸(SAM)辅因子的依赖性及酶动力学参数。
2. **文献名称**：*"Structural Insights into METTL11A-Mediated Methylation via Crystallography"*
**作者**：Tanaka K. et al.
**摘要**：解析了METTL11A重组蛋白的晶体结构，发现其催化结构域中保守的SAM结合口袋，并通过突变实验验证了关键残基对甲基转移活性的影响。
3. **文献名称**：*"METTL11A Regulates Ribosomal Protein Methylation and Cellular Stress Response"*
**作者**：Guo R. et al.
**摘要**：利用重组METTL11A进行体外甲基化筛选，鉴定出核糖体蛋白L12为新型底物，并证明其甲基化修饰在应激条件下调控蛋白质翻译过程。
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**注**：以上文献为假设性示例，实际研究中建议通过PubMed或Google Scholar以“METTL11A recombinant protein”“METTL11A methyltransferase”等关键词检索最新文献。若需真实文献，可提供进一步研究方向以缩小范围。
METTL11A, also known as N-α-acetyltransferase 60 (Naa60), is a member of the N-terminal acetyltransferase (NAT) family, which catalyzes the acetylation of proteins at their N-termini. This post-translational modification plays a critical role in regulating protein stability, localization, and interaction networks. Unlike most NATs that localize to the cytosol or nucleus, METTL11A is unique for its association with cellular membranes, particularly the Golgi apparatus and endosomes. It specifically acetylates the α-amino group of transmembrane proteins, influencing membrane trafficking, organelle dynamics, and cellular signaling pathways. Dysregulation of METTL11A has been linked to cancer progression, cardiovascular diseases, and neurological disorders, highlighting its biomedical relevance.
Recombinant METTL11A protein is produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable functional and structural studies. The purified protein retains enzymatic activity, allowing researchers to investigate substrate specificity, kinetic properties, and inhibitor screening in vitro. Its recombinant form is often tagged with affinity markers (e.g., His-tag, GST) for simplified purification and detection. Recent studies employ METTL11A recombinant protein to explore its role in membrane protein biology, including interactions with chaperones, modulation of receptor signaling, and involvement in pathological conditions like tumor angiogenesis. Additionally, it serves as a tool for developing targeted therapies aimed at modulating acetylation-dependent cellular processes. Research on METTL11A continues to uncover its regulatory mechanisms and potential as a diagnostic or therapeutic target.
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