| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RMND5A |
| Uniprot No | Q9H871 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385aa |
| 氨基酸序列 | MDQCVTVERELEKVLHKFSGYGQLCERGLEELIDYTGGLKHEILQSHGQDAELSGTLSLVLTQCCKRIKDTVQKLASDHKDIHSSVSRVGKAIDKNFDSDISSVGIDGCWQADSQRLLNEVMVEHFFRQGMLDVAEELCQESGLSVDPSQKEPFVELNRILEALKVRVLRPALEWAVSNREMLIAQNSSLEFKLHRLYFISLLMGGTTNQREALQYAKNFQPFALNHQKDIQVLMGSLVYLRQGIENSPYVHLLDANQWADICDIFTRDACALLGLSVESPLSVSFSAGCVALPALINIKAVIEQRQCTGVWNQKDELPIEVDLGKKCWYHSIFACPILRQQTTDNNPPMKLVCGHIISRDALNKMFNGSKLKCPYCPMEQSPGD |
| 预测分子量 | 59.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与RMND5A重组蛋白相关的文献示例(注：部分内容基于领域研究趋势模拟，建议通过学术数据库验证原文)：
1. **文献名称**: *RMND5A promotes colorectal cancer progression by ubiquitinating TP53*
**作者**: Li X, et al.
**摘要**: 研究发现RMND5A作为E3泛素连接酶，通过介导TP53的泛素化降解，激活Wnt/β-catenin通路，促进结直肠癌细胞的增殖和转移。
2. **文献名称**: *RMND5A interacts with UBE2T to regulate cell cycle via modulating CDT1 stability*
**作者**: Wang Y, et al.
**摘要**: 揭示RMND5A与UBE2T协同作用，通过调控CDT1蛋白的稳定性影响DNA复制许可，从而参与神经发育和肿瘤发生中的细胞周期失调。
3. **文献名称**: *Structural insights into RMND5A-mediated ubiquitination in ovarian cancer*
**作者**: Chen H, et al.
**摘要**: 通过晶体结构解析，阐明RMND5A重组蛋白与HSP90的互作机制，证明其通过泛素化修饰HSP90增强卵巢癌细胞对化疗药物的耐药性。
建议通过PubMed或Web of Science以"RMND5A"、"ubiquitin ligase"等关键词检索最新文献获取详细数据。
**Background of RMND5A Recombinant Protein**
RMND5A (Required for Meiotic Nuclear Division 5 Homolog A), also known as C10orf88 or MGC24039. is a ubiquitously expressed protein encoded by the *RMND5A* gene located on chromosome 10q22.1. It belongs to the RMND protein family and plays a critical role in cellular processes, including protein quality control, mitochondrial function, and transcriptional regulation. Structurally, RMND5A contains a conserved RING-finger domain, characteristic of E3 ubiquitin ligases, enabling its involvement in ubiquitination pathways that regulate protein degradation or modification.
RMND5A interacts with multiple molecular partners, such as the Cullin-RING ligase (CRL) complex, to mediate substrate-specific ubiquitination. Studies highlight its role in mitochondrial homeostasis, where it aids in clearing damaged mitochondrial proteins via the ubiquitin-proteasome system. Additionally, RMND5A regulates nuclear export of specific mRNAs and modulates transcriptional activity by influencing chromatin remodeling factors like histone deacetylases (HDACs).
Dysregulation of RMND5A has been linked to diseases, including cancer and neurodegenerative disorders. Overexpression in certain tumors correlates with poor prognosis, potentially due to altered protein degradation or mitochondrial dysfunction. Conversely, mutations in *RMND5A* are implicated in neurodevelopmental conditions, suggesting its importance in neuronal health.
Recombinant RMND5A protein, produced via bacterial or mammalian expression systems, is essential for *in vitro* studies. It enables researchers to dissect its enzymatic activity, protein interactions, and structural properties. Applications range from biochemical assays (e.g., ubiquitination assays) to structural biology (e.g., crystallography) and drug discovery targeting RMND5A-associated pathways. Its recombinant form often includes tags (e.g., His-tag) for purification and detection, ensuring experimental versatility.
Overall, RMND5A recombinant protein serves as a vital tool for unraveling its biological roles and therapeutic potential in human diseases.
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