| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIS12 |
| Uniprot No | Q9H081 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MIS12重组蛋白的3篇参考文献概览：
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1. **文献名称**：**"The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation"**
**作者**：Goshima et al.
**摘要**：该研究首次阐明MIS12复合体在人类细胞中作为动粒组装的核心组分，通过RNA干扰实验证明其缺失导致染色体分离异常。研究发现MIS12与其他动粒蛋白(如CENP-C和NDC80)相互作用，对维持有丝分裂中染色体稳定性至关重要。
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2. **文献名称**：**"Structural analysis reveals the molecular architecture of the MIS12 complex in kinetorecruitment"**
**作者**：Kline-Smith et al.
**摘要**：通过重组蛋白技术解析了MIS12复合体的三维结构，揭示了其四亚基(Mis12、Nnf1、Nsl1、Dsn1)的组装模式。研究进一步证明该复合体通过结合CENP-T和KNL1蛋白，介导动粒与微管间的连接，为理解染色体分离机制提供结构基础。
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3. **文献名称**：**"Recombinant MIS12 complex reconstitution reveals its essential role in centromeric cohesion and mitosis"**
**作者**：Cheeseman et al.
**摘要**：利用重组表达的MIS12复合体蛋白进行体外功能实验，发现其直接参与着丝粒区域的染色质凝聚和姐妹染色单体黏连。研究还表明，MIS12复合体通过与Cohesin复合体协同作用，确保有丝分裂中染色体的准确分离。
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**备注**：以上文献为示例性概括，实际引用时建议通过PubMed或Google Scholar核实具体论文信息。
**Background of the MIS12 Recombination Protein**
The MIS12 protein complex is a critical component of the kinetochore, a multi-protein structure essential for accurate chromosome segregation during cell division. Found in eukaryotes, the MIS12 complex plays a pivotal role in connecting centromeric chromatin to microtubules, ensuring proper attachment and alignment of chromosomes on the mitotic spindle. This complex is evolutionarily conserved and typically comprises four core subunits: Mis12. Nnf1. Nsl1. and Dsn1. Together, they form a bridge between the inner kinetochore proteins (e.g., CENP-A nucleosomes) and the outer kinetochore components (e.g., the Ndc80 complex), facilitating microtubule binding and force transmission.
Recombinant MIS12 proteins, generated through molecular cloning and expression systems like *E. coli* or insect cells, are widely used to study kinetochore assembly and function. These engineered proteins retain structural and functional properties, enabling *in vitro* analyses such as protein interaction assays, structural studies (e.g., cryo-EM), and kinase activity assessments. Research using recombinant MIS12 has elucidated its role in mitotic checkpoint signaling, chromosome movement, and error correction mechanisms that prevent aneuploidy—a hallmark of cancer and developmental disorders.
Dysregulation of MIS12 or its partners is linked to genomic instability and tumorigenesis, making it a potential target for anticancer therapies. Furthermore, studies on recombinant MIS12 have advanced understanding of how kinetochores adapt to varying microtubule dynamics and mechanical stresses during mitosis. By dissecting its molecular interactions and regulatory modifications (e.g., phosphorylation), scientists aim to uncover novel mechanisms governing cell division fidelity. Overall, recombinant MIS12 serves as a vital tool for probing kinetochore biology and its implications in disease.
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