| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAA38 |
| Uniprot No | Q9BRA0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-173aa |
| 氨基酸序列 | AVAVGVRAAPVLGLARALVLGLRGSQAARWRGWGTAAPGSLWALCECPAGCRELWFRGRAAWAARSERLLHPAQDSDGEREDSAAERARQQLEALLNKTMRIRMTDGRTLVGCFLCTDRDCNVILGSAQEFLKPSDSFSAGEPRVLGLAMVPGHHIVSIEVQRESLTGPPYL |
| 预测分子量 | 34.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于NAA38重组蛋白的参考文献示例(建议通过PubMed或Google Scholar核实真实文献)：
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1. **文献名称**: *"Structural insights into the human NatC complex reveal regulatory roles of NAA38 in N-terminal acetylation"*
**作者**: Smith J, et al.
**摘要**: 通过重组表达人源NAA38蛋白及其复合物，解析NatC乙酰转移酶的三维结构，证明NAA38通过稳定复合体构象增强底物识别能力，并揭示其在癌症细胞增殖中的调控作用。
2. **文献名称**: *"Functional characterization of recombinant NAA38 in Drosophila models reveals evolutionary conservation of NatC activity"*
**作者**: Lee H, et al.
**摘要**: 利用重组NAA38蛋白在果蝇模型中验证其功能保守性，证明其与NAA35/NAA30协同调控胚胎发育，并通过体外实验证实重组蛋白可恢复NatC缺陷细胞的乙酰化活性。
3. **文献名称**: *"Optimization of NAA38 recombinant protein production in mammalian cells for therapeutic target screening"*
**作者**: Gupta R, et al.
**摘要**: 开发哺乳动物细胞表达系统高效生产重组NAA38蛋白，优化纯化工艺并应用于高通量药物筛选，发现小分子抑制剂可阻断NatC依赖的肿瘤细胞迁移。
4. **文献名称**: *"NAA38 interacts with viral proteins and modulates host antiviral response: evidence from recombinant protein-based assays"*
**作者**: Zhang X, et al.
**摘要**: 通过重组NAA38蛋白的体外结合实验，发现其与特定病毒蛋白相互作用，抑制宿主细胞NatC活性，并提出其作为抗病毒治疗潜在靶点的可能性。
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**注意**：以上文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)以关键词 **"NAA38 recombinant"** 或 **"NatC complex NAA38"** 检索真实文献。
**Background of NAA38 Recombinant Protein**
NAA38. also known as N-alpha-acetyltransferase 38. is a subunit of the NatA complex, a major eukaryotic N-terminal acetyltransferase responsible for co-translational acetylation of proteins. This post-translational modification plays a critical role in regulating protein stability, localization, and interactions, impacting diverse cellular processes such as cell cycle progression, apoptosis, and stress responses. The NatA complex comprises the catalytic subunit Naa10 (ARD1) and the auxiliary subunit Naa15 (NAT1), with NAA38 (also called HYPK or Huntingtin yeast partner K) acting as a stabilizing factor essential for complex integrity and enzymatic activity.
NAA38 recombinant protein is generated through genetic engineering, typically expressed in bacterial or mammalian systems, to study its structure, function, and interactions. Recombinant versions allow researchers to explore its role in NatA-mediated acetylation and its broader biological implications. Studies have shown that NAA38 depletion disrupts NatA activity, leading to developmental defects in model organisms, such as zebrafish embryos, and altered stress responses in cell lines.
Furthermore, NAA38 has been linked to diseases, including cancer and neurodegenerative disorders. Dysregulation of NatA activity, influenced by NAA38 expression, is observed in tumors, where it may promote proliferation or metastasis. In neurodegenerative contexts, NAA38’s interaction with Huntingtin (HTT) suggests a potential role in modulating Huntington’s disease pathology.
Research utilizing NAA38 recombinant protein aids in deciphering its molecular mechanisms, developing biochemical assays, and screening for therapeutic agents targeting acetylation pathways. Its study contributes to understanding the intricate regulation of protein acetylation and its implications in health and disease.
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