| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHIA |
| Uniprot No | Q9BZP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368aa |
| 氨基酸序列 | MVSTPENRQTFITSVIKFLRQYEFDGLDFDWEYPGSRGSPPQDKHLFTVLVQEMREAFEQEAKQINKPRLMVTAAVAAGISNIQSGYEIPQLSQYLDYIHVMTYDLHGSWEGYTGENSPLYKYPTDTGSNAYLNVDYVMNYWKDNGAPAEKLIVGFPTYGHNFILSNPSNTGIGAPTSGAGPAGPYAKESGIWAYYEICTFLKNGATQGWDAPQEVPYAYQGNVWVGYDNIKSFDIKAQWLKHNKFGGAMVWAIDLDDFTGTFCNQGKFPLISTLKKALGLQSASCTAPAQPIEPITAAPSGSGNGSGSSSSGGSSGGSGFCAVRANGLYPVANNRNAFWHCVNGVTYQQNCQAGLVFDTSCDCCNWA |
| 预测分子量 | 67.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于CHIA(几丁质酶)重组蛋白研究的虚构参考文献示例(内容根据常见研究方向模拟，非真实文献)：
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1. **文献名称**: *Expression and characterization of recombinant human CHIA in Escherichia coli*
**作者**: Zhang L., et al.
**摘要**: 研究通过大肠杆菌表达系统成功重组表达人源CHIA蛋白，优化诱导条件后获得可溶性蛋白，并证实其具有水解几丁质底物的活性，为后续功能研究奠定基础。
2. **文献名称**: *Functional analysis of plant-derived CHIA in fungal resistance*
**作者**: Tanaka K., et al.
**摘要**: 通过重组表达拟南芥CHIA蛋白，发现其可显著抑制病原真菌菌丝生长，揭示其在植物先天免疫中的关键作用，为农业抗病基因工程提供理论依据。
3. **文献名称**: *Structural insights into CHIA catalytic mechanism by recombinant protein crystallization*
**作者**: Müller R., et al.
**摘要**: 利用昆虫细胞系统表达高纯度CHIA重组蛋白，成功解析其晶体结构，结合突变实验阐明底物结合域和催化活性位点的分子机制。
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注：以上文献为模拟示例，实际研究需通过学术数据库(如PubMed/Web of Science)检索关键词“chitinase recombinant expression”或结合具体物种名称查询。
Chia (Chitinase Acidic) is a mammalian enzyme belonging to the glycosyl hydrolase family 18 (GH18), primarily known for its ability to hydrolyze chitin, a structural polysaccharide found in fungi, insects, and parasites. Unlike plants or invertebrates, mammals do not synthesize chitin endogenously, but Chia plays a critical role in innate immunity by breaking down exogenous chitin-containing pathogens. In humans, CHIA is expressed in tissues exposed to environmental antigens, such as the lungs, gastrointestinal tract, and salivary glands. Its activity is linked to immune modulation, particularly in allergic responses and asthma, where dysregulated chitinase activity may exacerbate inflammation.
Recombinant CHIA proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to study their structural and functional properties. These proteins retain chitinolytic activity and are utilized to investigate host-pathogen interactions, chitin metabolism, and their role in inflammatory diseases. Structural studies of recombinant CHIA have revealed conserved catalytic domains and substrate-binding regions, aiding in the design of inhibitors or therapeutic agents.
Research applications include exploring CHIA's potential in immunotherapy, such as targeting fungal infections or modulating Th2-driven allergies. Additionally, recombinant CHIA serves as a tool to dissect its physiological roles, including tissue remodeling and immune surveillance. Challenges remain in optimizing stability and activity for clinical use, but advances in protein engineering continue to expand its biomedical relevance. Understanding CHIA's molecular mechanisms may pave the way for novel treatments for chitin-related pathologies and immune disorders.
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