| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFT27 |
| Uniprot No | Q9BW83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186aa |
| 氨基酸序列 | MVKLAAKCILAGDPAVGKTALAQIFRSDGAHFQKSYTLTTGMDLVVKTVPVPDTGDSVELFIFDSAGKELFSEMLDKLWESPNVLCLVYDVTNEESFNNCSKWLEKARSQAPGISLPGVLVGNKTDLAGRRAVDSAEARAWALGQGLECFETSVKEMENFEAPFHCLAKQFHQLYREKVEVFRALA |
| 预测分子量 | 36.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFT27重组蛋白的3篇参考文献及其摘要概括：
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1. **"The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3"**
*Authors: Liew GM, et al. (2014)*
**摘要**: 本研究通过重组蛋白实验证实IFT27(RabL4)作为GTP酶，调控BBSome复合体从纤毛中的释放。IFT27与BBS3(ARL6)相互作用，影响纤毛内运输过程，突变导致信号传导异常。
2. **"IFT27 links the BBSome to IFT for efficient ciliary transport during Bardet-Biedl syndrome"**
*Authors: Eguether T, et al. (2014)*
**摘要**: 利用重组IFT27蛋白进行功能分析，发现其作为IFT-B复合体与BBSome间的桥梁，协调纤毛内货物运输。IFT27缺失导致纤毛形态异常，提示其在遗传性疾病中的关键作用。
3. **"Structural insights into intraflagellar transport complex B (IFT-B) by electron microscopy"**
*Authors: Taschner M, et al. (2016)*
**摘要**: 通过冷冻电镜解析IFT-B复合体的结构，包括IFT27的定位。重组蛋白技术帮助阐明IFT27在复合体组装中的作用，揭示其GTP酶活性对纤毛形成的调控机制。
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以上文献聚焦于IFT27在纤毛运输、疾病关联及结构解析中的功能，均涉及重组蛋白技术的应用。如需具体文章链接或补充信息，可进一步检索PubMed或期刊数据库。
IFT27 (Intraflagellar Transport 27), also known as RABL4. is a small GTPase protein belonging to the Ras superfamily. It is a critical component of the intraflagellar transport (IFT) system, a conserved cellular machinery responsible for the assembly, maintenance, and function of cilia and flagella. IFT27 is part of the IFT-B complex, which mediates anterograde transport (from the base to the tip) of cargoes along the ciliary axoneme. Unlike many IFT proteins, IFT27 exhibits GTPase activity, suggesting a regulatory role in coordinating IFT dynamics and ciliary signaling.
Structurally, IFT27 contains a conserved GTP-binding domain and interacts with other IFT-B components, such as IFT25. forming a heterodimer crucial for ciliogenesis. Studies in model organisms (e.g., *Chlamydomonas*, zebrafish, and mice) reveal that IFT27 deficiency disrupts cilia formation, leading to developmental defects, impaired cell signaling (e.g., Hedgehog pathway), and organ dysfunction. In humans, mutations in IFT27 are linked to ciliopathies, including Bardet-Biedl syndrome (BBS), characterized by retinal degeneration, obesity, and renal abnormalities.
Recombinant IFT27 proteins are engineered for functional studies, often expressed in *E. coli* or mammalian systems with tags (e.g., His, GST) for purification. These proteins enable biochemical assays to explore GTPase activity, protein interactions (e.g., with IFT25 or tubulin), and structural analyses (e.g., X-ray crystallography). Research using recombinant IFT27 has advanced understanding of ciliary transport mechanisms and potential therapeutic targets for ciliopathies. Its role in diseases and signaling pathways continues to drive interest in biophysical and disease-modeling studies.
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