| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASB6 |
| Uniprot No | Q9NWX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MPFLHGFRRIIFEYQPLVDAILGSLGIQDPERQESLDRPSYVASEESRILVLTELLERKAHSPFYQEGVSNALLKMAELGLTRAADVLLRHGANLNFEDPVTYYTALHIAVLRNQPDMVELLVHHGADVNRRDREKLLCSMLWPAATGCRSTILRTFVSYWKEGQTSRPPPKMGTQCSPASSSCLVRPWEGTKRRPR |
| 预测分子量 | 38.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASB6重组蛋白的3篇参考文献示例(注：内容为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*Recombinant Expression and Functional Analysis of ASB6 in Ubiquitination Pathways*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了ASB6重组蛋白，证实其通过SOCS结构域与Elongin B/C形成复合物，并作为E3泛素连接酶调控特定底物的泛素化降解。
2. **文献名称**：*Structural Characterization of ASB6 and Its Interaction with Cellular Signaling Proteins*
**作者**：Li Y, Wang X.
**摘要**：利用哺乳动物细胞表达系统获得ASB6重组蛋白，结合X射线晶体学解析其三维结构，揭示了其ANK重复结构域与TSC2蛋白的相互作用，提示其在mTOR信号通路中的潜在作用。
3. **文献名称**：*ASB6 Knockdown and Recombinant Rescue in Cancer Cell Proliferation Studies*
**作者**：Garcia R, et al.
**摘要**：通过重组ASB6蛋白回补实验，验证了ASB6在抑制结直肠癌细胞增殖中的功能，并证明其依赖泛素化修饰调控细胞周期蛋白稳定性。
建议通过PubMed或Google Scholar搜索关键词“ASB6 recombinant”或“ASB6 ubiquitin”获取真实文献。
**Background of ASB6 Recombinant Protein**
ASB6 (Ankyrin repeat and SOCS box protein 6) is a member of the ASB family, characterized by a unique structural organization comprising ankyrin repeat domains and a C-terminal SOCS (suppressor of cytokine signaling) box. The ankyrin repeats mediate protein-protein interactions, while the SOCS box facilitates the assembly of E3 ubiquitin ligase complexes, implicating ASB6 in substrate recognition and proteasomal degradation pathways. This dual-domain architecture suggests a role in cellular processes such as signal transduction, protein turnover, and regulation of metabolic or inflammatory pathways.
The ASB6 gene is conserved across vertebrates, with studies linking its expression to tissues like the brain, heart, and skeletal muscle. Although its precise physiological functions remain under investigation, ASB6 is hypothesized to influence cell proliferation, apoptosis, and energy homeostasis. Dysregulation of ASB family proteins has been associated with cancers, neurodegenerative disorders, and metabolic syndromes, positioning ASB6 as a potential biomarker or therapeutic target.
Recombinant ASB6 protein is typically produced using expression systems (e.g., *E. coli*, mammalian cells*) to enable functional and structural studies. Purification tags (e.g., His-tag) are often incorporated to streamline isolation. Researchers employ this tool to explore ASB6’s interactome, substrate specificity, and mechanistic roles in ubiquitination cascades. Its recombinant form also aids in antibody development, high-throughput screening, and elucidating pathological mechanisms in disease models.
Overall, ASB6 recombinant protein serves as a critical reagent for advancing understanding of ubiquitin-mediated regulatory networks and their implications in health and disease.
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