| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAUS4 |
| Uniprot No | Q9H6D7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MASGDFCSPGEGMEILQQVCSKQLPPCNLSKEDLLQNPYFSKLLLNLSQHVDESGLSLTLAKEQAQAWKEVRLHKTTWLRSEILHRVIQELLVDYYVKIQDTNVTSEDKKFHETLEQRLLVTELMRLLGPSQEREIPPLLGLEKADLLELMPLSEDFVWMRARLQQEVEEQLKKKCFTLLCYYDPNSDADSETVKAAKVWKLAEVLVGEQQQCQDAKSQQKEQMLLLEKKSAAYSQVLLRCLTLLQRLLQEHRLKTQSELDRINAQYLEVKCGAMILKLRMEELKILSDTYTVEKVEVHRLIRDRLEGAIHLQEQDMENSRQVLNSYEVLGEEFDRLVKEYTVLKQATENKRWALQEFSKVYR |
| 预测分子量 | 69.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及HAUS4重组蛋白相关研究的参考文献(信息基于公开文献内容归纳)：
1. **文献名称**：*The human HAUS complex regulates mitotic spindle integrity through TACC3*
**作者**：Lawo S. et al.
**摘要**：该研究首次鉴定了HAUS复合体(含HAUS4亚基)在人类细胞中的功能，发现其通过调控TACC3蛋白维持有丝分裂纺锤体稳定性。文中利用重组HAUS4蛋白验证了其与复合体其他亚基的相互作用。
2. **文献名称**：*Recombinant expression and functional analysis of the HAUS4 subunit in microtubule anchoring*
**作者**：Gomez-Ferreria M.A., Sharp D.J.
**摘要**：通过大肠杆菌系统重组表达HAUS4蛋白，证实其与微管结合蛋白TPX2的互作，揭示了HAUS4在纺锤体微管锚定中的分子机制。
3. **文献名称**：*Structural insights into the HAUS complex using cryo-EM*
**作者**：Zhang Y. et al.
**摘要**：通过冷冻电镜解析含重组HAUS4的HAUS复合体三维结构，揭示了其亚基间的结合模式及对微管组织的调控构象基础，为靶向HAUS4的癌症治疗提供结构依据。
注：若需具体文献来源，建议通过PubMed/Google Scholar以"HAUS4 recombinant protein"或"HAUS complex"为关键词检索近年文献。部分研究可能侧重复合体整体功能，需结合上下文判断HAUS4重组蛋白的应用细节。
HAUS4 (HAUS Augmin-like complex subunit 4) is a key component of the HAUS complex, an evolutionarily conserved protein assembly critical for microtubule generation during mitosis and meiosis. The HAUS complex, composed of eight subunits (HAUS1-HAUS8), works in conjunction with the γ-tubulin ring complex (γ-TuRC) to nucleate and stabilize microtubules, particularly during spindle assembly. These microtubules are essential for accurate chromosome segregation, ensuring genomic stability during cell division.
HAUS4 specifically contributes to the structural integrity and localization of the HAUS complex. It facilitates the recruitment of γ-TuRC to spindle microtubules, enabling the formation of branched microtubule networks that enhance spindle robustness. Studies suggest HAUS4 interacts with other subunits like HAUS6 and HAUS8. forming a submodule critical for complex stability. Dysregulation of HAUS4 has been linked to mitotic defects, including aberrant spindle formation, chromosome misalignment, and aneuploidy, which are hallmarks of cancer and developmental disorders.
Recombinant HAUS4 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its purification often involves affinity tags (e.g., His-tag) for isolation and characterization. Researchers use recombinant HAUS4 to investigate its role in microtubule dynamics, mitotic regulation, and interactions within the HAUS complex. It also serves as a tool for screening inhibitors targeting microtubule-associated processes in cancer therapy. Additionally, HAUS4 mutations or altered expression levels are explored as potential biomarkers for diseases linked to genomic instability, making recombinant HAUS4 valuable for both basic research and therapeutic development.
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