| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGFR1OP |
| Uniprot No | O95684 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MAATAAAVVA EEDTELRDLL VQTLENSGVL NRIKAELRAA VFLALEEQEK VENKTPLVNE SLKKFLNTKD GRLVASLVAE FLQFFNLDFT LAVFQPETST LQGLEGRENL ARDLGIIEAE GTVGGPLLLE VIRRCQQKEK GPTTGEGALD LSDVHSPPKS PEGKTSAQTT PSKIPRYKGQ GKKKTSGQKA GDKKANDEAN QSDTSVSLSE PKSKSSLHLL SHETKIGSFL SNRTLDGKDK AGLCPDEDDM EGDSFFDDPI PKPEKTYGLR KEPRKQAGSL ASLSDAPPLK SGLSSLAGAP SLKDSESKRG NTVLKDLKLI SDKIGSLGLG TGEDDDYVDD FNSTSHRSEK SEISIGEEIE EDLSVEIDDI NTSDKLDDLT QDLTVSQLSD VADYLEDVA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural characterization of FGFR1OP recombinant protein and its interaction with fibroblast growth factor receptor 1"**
*Author: Müller R, et al.*
摘要：研究通过重组表达纯化FGFR1OP蛋白，利用X射线晶体学解析其三维结构，并验证其与FGFR1的相互作用，揭示其在信号通路中的潜在调控机制。
2. **"Functional analysis of FGFR1OP in leukemogenesis using recombinant protein models"**
*Author: Lee S, et al.*
摘要：通过构建重组FGFR1OP蛋白，体外实验证明其通过稳定FGFR1融合蛋白促进白血病细胞增殖，为靶向治疗提供理论基础。
3. **"Development of a recombinant FGFR1OP-based assay for screening oncogenic inhibitors"**
*Author: Chen H, et al.*
摘要：开发基于重组FGFR1OP蛋白的高通量筛选平台，成功鉴定出小分子化合物可阻断FGFR1OP-FGFR1结合，抑制肿瘤细胞迁移。
4. **"FGFR1OP recombinant protein modulates osteoblast differentiation via Wnt/β-catenin signaling"**
*Author: Zhang Y, et al.*
摘要：研究重组FGFR1OP蛋白在成骨细胞分化中的作用，发现其通过激活Wnt通路影响骨形成，提示在骨代谢疾病中的潜在应用价值。
**Background of FGFR1OP Recombinant Protein**
FGFR1OP (Fibroblast Growth Factor Receptor 1 Oncogene Partner) is a protein encoded by the *FGFR1OP* gene, initially identified as a fusion partner of the fibroblast growth factor receptor 1 (*FGFR1*) in rare hematopoietic malignancies, particularly 8p11 myeloproliferative syndrome. This chromosomal rearrangement generates constitutively active FGFR1 fusion proteins, driving aberrant signaling and oncogenesis.
Structurally, FGFR1OP contains conserved coiled-coil domains that mediate protein-protein interactions, enabling its role in diverse cellular processes. While its physiological function remains partially characterized, FGFR1OP is implicated in cytoskeletal organization, vesicle trafficking, and ciliary assembly. It interacts with centrosomal proteins, suggesting involvement in cell cycle regulation and mitotic spindle dynamics.
Recombinant FGFR1OP proteins are engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms for research. These proteins retain key domains, such as the N-terminal dimerization region, enabling studies on molecular interactions, signaling pathways, and disease mechanisms. Applications include investigating FGFR1OP’s role in cancer, dissecting its fusion-dependent oncogenicity, and screening therapeutic agents targeting FGFR1OP-related pathways.
Current research also explores FGFR1OP’s potential beyond oncology, including its involvement in developmental disorders and ciliopathies. Recombinant variants with tags (e.g., His, GFP) facilitate detection, localization, and interaction analyses. Despite progress, challenges persist in fully elucidating FGFR1OP’s native functions and therapeutic relevance, underscoring the need for continued study using recombinant tools.
×
