| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXO5 |
| Uniprot No | Q9H790 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MAETREEETVSAEASGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWKRGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFGEGEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYADICEWRKGSGVLSSTLAPQVKKAK |
| 预测分子量 | 57.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXO5重组蛋白的虚构参考文献示例，供参考格式使用：
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1. **标题**：Cloning and Functional Characterization of Recombinant EXO5 in *E. coli*
**作者**：Kim J, Zhang L, Park S
**摘要**：本研究报道了EXO5基因的克隆及其在大肠杆菌中的高效可溶性表达。通过亲和层析纯化获得重组蛋白，并验证其在体外具有DNA结合和核酸酶活性，为DNA修复机制研究提供工具。
2. **标题**：Structural Insights into EXO5 Recombinant Protein by Cryo-EM
**作者**：Wang Y, Tanaka A, Li R
**摘要**：利用冷冻电镜解析EXO5重组蛋白的3.1 Å分辨率结构，揭示其底物结合域的关键氨基酸残基，为设计靶向EXO5功能的抑制剂奠定结构基础。
3. **标题**：EXO5 Recombinant Protein Enhances CRISPR-Cas9 Genome Editing Efficiency
**作者**：Chen H, Müller P, Wei X
**摘要**：研究发现，纯化的EXO5重组蛋白可通过调控DNA末端切除提升CRISPR-Cas9系统的同源定向修复效率，为基因编辑技术优化提供新策略。
4. **标题**：Role of Recombinant EXO5 in Mitochondrial DNA Repair Pathways
**作者**：Gomez F, Silva D, Rossi M
**摘要**：通过体外实验证实，EXO5重组蛋白特异性识别并修复线粒体DNA氧化损伤位点，提示其在维持线粒体基因组稳定性中的潜在作用。
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注：以上文献为模拟内容，实际研究中请根据具体课题检索权威数据库(如PubMed)。
**Background of EXO5 Recombinant Protein**
EXO5. also known as Exonuclease 5. is a structure-specific DNA exonuclease implicated in critical genomic maintenance processes, particularly in DNA repair pathways. It belongs to the exonuclease family of enzymes that catalyze the removal of nucleotides from DNA strands, playing a role in resolving DNA damage, replication stress, and recombination intermediates. EXO5 specifically recognizes and processes branched DNA structures, such as 5' overhangs or flap structures, making it essential for repairing single-strand breaks and maintaining genomic stability.
The recombinant form of EXO5 (EXO5 recombinant protein) is engineered using molecular cloning techniques, typically expressed in prokaryotic systems like *E. coli* or eukaryotic systems to ensure proper folding and post-translational modifications. This recombinant protein retains the enzymatic activity of its native counterpart, enabling researchers to study its biochemical properties, substrate specificity, and interaction partners in vitro. Its production often involves affinity chromatography purification, ensuring high purity and stability for experimental applications.
Structurally, EXO5 contains conserved catalytic domains critical for its exonuclease activity. Studies highlight its role in base excision repair (BER), a pathway addressing small base lesions, and its potential interplay with proteins like PARP1 or XRCC1. Dysregulation of EXO5 has been linked to genomic instability and diseases, including cancer, underscoring its biological relevance.
In research, recombinant EXO5 is utilized to dissect DNA repair mechanisms, screen for inhibitors, or develop diagnostic tools. Its recombinant form offers scalability and reproducibility, bypassing challenges associated with isolating the protein from native tissues. Further investigations into EXO5 may uncover therapeutic targets for cancer or neurodegenerative disorders tied to defective DNA repair.
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