| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL20 |
| Uniprot No | Q9BYC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 46-149aa |
| 氨基酸序列 | VIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH |
| 预测分子量 | 27.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于MRPL20重组蛋白的模拟参考文献(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**: *Functional characterization of MRPL20 in mitochondrial ribosome assembly*
**作者**: Smith A, et al.
**摘要**: 本研究通过重组表达人源MRPL20蛋白，发现其在线粒体核糖体大亚基组装中起关键作用。利用CRISPR敲除细胞模型，证实MRPL20缺失导致线粒体翻译功能受损，并引发氧化磷酸化缺陷。
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2. **文献名称**: *Crystal structure of recombinant MRPL20 reveals conserved ribosomal protein motifs*
**作者**: Tanaka K, et al.
**摘要**: 作者解析了重组MRPL20蛋白的晶体结构，揭示了其与细菌同源蛋白L20的结构相似性，并鉴定了参与线粒体rRNA结合的特定结构域，为理解核糖体进化提供了新视角。
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3. **文献名称**: *MRPL20 mutations linked to mitochondrial encephalopathy: In vitro reconstitution and functional analysis*
**作者**: Chen L, et al.
**摘要**: 通过重组表达携带临床突变的MRPL20蛋白，发现特定突变(如p.R123C)破坏其与线粒体16S rRNA的相互作用，导致核糖体亚基组装异常，解释了患者神经退行性表型的分子机制。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“MRPL20 recombinant protein”或“MRPL20 mitochondrial ribosome”获取。
**Background of MRPL20 Recombinant Protein**
MRPL20 (Mitochondrial Ribosomal Protein L20) is a component of the large subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translation machinery to produce subunits of oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP generation. MRPL20. encoded by nuclear DNA, is imported into mitochondria and assembled into the 39S ribosomal subunit. Its role in maintaining ribosomal stability and facilitating interactions with mitochondrial rRNA highlights its importance in mitochondrial function.
Recombinant MRPL20 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable large-scale production for functional studies. Researchers utilize this protein to investigate mitochondrial translation mechanisms, ribosome assembly, and defects linked to mitochondrial disorders. Mutations or dysregulation in MRPL20 have been implicated in diseases such as cancer, neurodegenerative conditions, and metabolic syndromes, where mitochondrial dysfunction is a hallmark.
Additionally, recombinant MRPL20 serves as a tool for structural studies (e.g., cryo-EM) to map ribosome architecture and identify drug targets. Its applications extend to diagnostics, where altered expression levels may act as biomarkers for mitochondrial pathologies. By studying MRPL20. scientists aim to uncover therapeutic strategies to restore mitochondrial homeostasis in disease contexts.
In summary, MRPL20 recombinant protein bridges fundamental research and clinical insights, advancing our understanding of mitochondrial biology and its role in human health.
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