| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKTIP |
| Uniprot No | Q9H8T0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT |
| 预测分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **AKTIP重组蛋白** 的3条示例参考文献(内容为示例，非真实文献)：
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1. **文献名称**：*"AKTIP重组蛋白的克隆表达及其在AKT信号通路中的功能研究"*
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究通过基因工程技术成功克隆并表达重组AKTIP蛋白，发现其与AKT1激酶相互作用，调控细胞凋亡和增殖，为AKT信号通路的分子机制提供了新见解。
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2. **文献名称**：*"AKTIP重组蛋白在乳腺癌细胞迁移中的泛素化调控作用"*
**作者**：Sato K, Gupta R, et al.
**摘要**：通过纯化重组AKTIP蛋白，揭示了其作为E3泛素连接酶复合物组分，通过泛素化修饰靶蛋白促进乳腺癌细胞迁移和侵袭的分子机制。
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3. **文献名称**：*"AKTIP重组蛋白的结构解析及其与病毒蛋白的互作研究"*
**作者**：Martinez D, Liu X, et al.
**摘要**：利用X射线晶体学解析了重组AKTIP蛋白的三维结构，发现其N端结构域与HPV E6蛋白结合，可能参与病毒致癌过程。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“AKTIP recombinant protein”或“AKTIP ubiquitination”。
**AKTIP Recombinant Protein: Background and Significance**
AKTIP (AKT Interacting Protein), also known as FIP3 or Rabin8. is a multifunctional protein involved in regulating intracellular signaling pathways, vesicular trafficking, and cell cycle progression. It interacts directly with AKT (Protein Kinase B), a serine/threonine kinase central to the PI3K-AKT-mTOR signaling axis, which governs critical cellular processes such as survival, proliferation, and metabolism. AKTIP’s association with AKT modulates AKT activation and downstream signaling, influencing cell growth and apoptosis. Beyond its role in AKT signaling, AKTIP acts as a guanine nucleotide exchange factor (GEF) for Rab8. a small GTPase essential for membrane trafficking and ciliogenesis, linking it to organelle dynamics and primary cilium formation.
Recombinant AKTIP protein is engineered through genetic recombination techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This purified protein retains functional domains critical for AKT binding, Rab8 activation, and interaction with other partners like centrosomal proteins. Its production enables researchers to study AKTIP’s biochemical properties, structure-function relationships, and regulatory mechanisms in vitro.
Studies highlight AKTIP’s dual roles in cancer biology. While it may promote tumor growth by enhancing AKT signaling, its involvement in ciliogenesis suggests potential tumor-suppressive functions in certain contexts. Dysregulation of AKTIP has been implicated in cancers, ciliopathies, and neurological disorders. Recombinant AKTIP serves as a vital tool for elucidating these mechanisms, screening therapeutic compounds, and developing targeted therapies. Additionally, it aids in exploring AKTIP’s contributions to cellular homeostasis, making it a valuable resource for both basic research and translational applications.
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