| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEAD3 |
| Uniprot No | Q99594 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 112-435aa |
| 氨基酸序列 | MNLDQVSKDKALQSMASMSSAQIVSASVLQNKFSPPSPLPQAVFSTSSRFWSSPPLLGQQPGPSQDIKPFAQPAYPIQPPLPPTLSSYEPLAPLPSAAASVPVWQDRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTISVSTKVCSFGKQVVEKVETEYARLENGRFVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTSRDSQETLLVIAFVFEVSTSEHGAQHHVYKLVKD |
| 预测分子量 | 43.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TEAD3重组蛋白的3篇示例参考文献(注：部分内容可能基于已有研究的概括整合，建议通过学术数据库核实具体文献)：
1. **标题**：*"Crystal structure of human TEAD3 in complex with YAP reveals key interactions for Hippo pathway regulation"*
**作者**：Zhou, X., et al.
**摘要**：通过重组表达纯化的TEAD3蛋白，解析了其与YAP共激活因子的复合物结构，揭示了二者结合的关键界面，为靶向TEAD-YAP相互作用的小分子设计提供了结构基础。
2. **标题**：*"Functional characterization of TEAD3 in trophoblast differentiation using recombinant protein mutagenesis"*
**作者**：Zhang, Y., et al.
**摘要**：研究利用重组TEAD3蛋白进行体外功能实验，发现其DNA结合结构域的特定突变会破坏胎盘滋养层细胞的分化，表明TEAD3在胚胎发育中的重要作用。
3. **标题**：*"Recombinant TEAD3 directly binds to M-CAT elements and regulates muscle-specific gene expression"*
**作者**：Jiang, L., et al.
**摘要**：通过电泳迁移实验(EMSA)验证重组TEAD3蛋白特异性结合M-CAT顺式调控元件，并激活肌肉相关基因的转录，证明其在肌肉分化中的直接调控功能。
4. **标题**：*"Development of a TEAD3 recombinant protein-based assay for high-throughput drug screening in cancer"*
**作者**：Chen, H., et al.
**摘要**：研究构建了重组TEAD3蛋白的体外荧光素酶报告系统，用于筛选抑制TEAD3转录活性的化合物，为靶向Hippo通路异常激活的癌症治疗提供新工具。
**注意**：以上文献名称及作者为示例性质，实际研究请以具体学术数据库检索结果为准。
**Background of TEAD3 Recombinant Protein**
TEAD3 (Transcriptional Enhanced Associate Domain 3) is a member of the TEAD family of transcription factors, which includes four paralogs (TEAD1–4) in vertebrates. These proteins play pivotal roles in development, cell proliferation, and tissue homeostasis by regulating genes involved in these processes. TEAD proteins function through interactions with co-activators such as YAP (Yes-associated protein) and TAZ (Transcriptional co-activator with PDZ-binding motif), which are central effectors of the Hippo signaling pathway. Dysregulation of TEAD-mediated transcription is implicated in cancers, fibrosis, and other diseases.
TEAD3 is distinct in its tissue-specific expression, predominantly found in the placenta, testis, and specific embryonic tissues. It binds to conserved MCAT motifs in DNA via its TEA domain, enabling the recruitment of co-activators to drive target gene expression. Structural studies reveal that TEAD3. like other TEADs, contains a conserved β-strand-rich DNA-binding domain and a palmitate-binding pocket critical for protein stability and interactions.
Recombinant TEAD3 protein is engineered for in vitro studies to dissect its biochemical and functional properties. Produced using bacterial or mammalian expression systems, it retains the ability to bind DNA and interact with co-factors. Researchers utilize purified TEAD3 to study its role in Hippo pathway signaling, investigate its oncogenic potential, and screen for inhibitors that disrupt TEAD-coactivator interactions—a therapeutic strategy for cancers driven by Hippo pathway dysregulation.
The development of TEAD3 recombinant protein has advanced mechanistic insights into its transcriptional regulation and facilitated drug discovery efforts targeting TEAD-dependent pathologies. Its applications span structural biology, molecular interaction assays, and high-throughput screening platforms.
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