| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPIN2B |
| Uniprot No | Q9BPZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258aa |
| 氨基酸序列 | MKTPNAQEAEGQQTRAAAGRATGSANMTKKKVSQKKQRGRPSSQPRRNIVGCRISHGWKEGDEPITQWKGTVLDQVPINPSLYLVKYDGIDCVYGLELHRDERVLSLKILSDRVASSHISDANLANTIIGKAVEHMFEGEHGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTEREPGGVVDGLIGKHVEYTKEDGSKRIGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKKS |
| 预测分子量 | 56.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SPIN2B重组蛋白的模拟参考文献(实际文献请通过学术数据库核实)：
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1. **文献名称**: *"Expression and purification of recombinant human SPIN2B protein in Escherichia coli"*
**作者**: Chen X, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统高效生产人源SPIN2B重组蛋白的优化方法，采用His标签纯化技术获得高纯度蛋白，并验证其通过Western blot的特异性识别。
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2. **文献名称**: *"Structural insights into SPIN2B's role in chromatin remodeling through HDAC interaction"*
**作者**: Li Y, Wang R.
**摘要**: 通过X射线晶体学解析SPIN2B重组蛋白的三维结构，揭示其与组蛋白去乙酰化酶(HDAC)复合物的结合模式，提出SPIN2B可能通过表观遗传调控影响基因沉默的分子机制。
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3. **文献名称**: *"Functional characterization of SPIN2B in mouse embryonic stem cell pluripotency"*
**作者**: Zhang L, et al.
**摘要**: 利用重组SPIN2B蛋白进行体外实验，证明其通过调控Nanog等关键转录因子的活性维持胚胎干细胞的自我更新，并证实其C端结构域对功能至关重要。
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**注意**：以上内容为基于研究领域常见主题的模拟生成，实际文献请通过PubMed、Web of Science或Google Scholar等平台检索。
**Background of SPIN2B Recombinant Protein**
SPIN2B (Spindlin Family Member 2B) is a member of the SPIN/SSTY protein family, characterized by a conserved N-terminal domain and tandem Tudor-like "SPIN" domains. It is primarily expressed in germ cells and plays a role in gametogenesis, particularly during spermatogenesis. Structurally, SPIN2B contains a lysine-tryptophan (KW) repeat domain, which facilitates interactions with chromatin-modifying complexes, suggesting its involvement in epigenetic regulation.
Studies indicate that SPIN2B may act as a histone code reader, binding to specific post-translational modifications (e.g., H3K4me3) to influence chromatin remodeling and transcriptional regulation. Its expression is tightly regulated during development, with dysregulation linked to reproductive disorders and certain cancers. For instance, SPIN2B overexpression has been observed in testicular germ cell tumors, highlighting its potential oncogenic role.
Recombinant SPIN2B protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. This engineered protein retains native folding and biochemical activity, allowing researchers to investigate its interactions with chromatin, role in cell cycle progression, and impact on stem cell pluripotency. Applications include *in vitro* binding assays, antibody development, and screening for small-molecule inhibitors targeting its chromatin-binding domains.
Current research focuses on elucidating SPIN2B's molecular mechanisms in reproduction and disease, positioning it as a potential biomarker or therapeutic target. Its recombinant form remains a critical tool for advancing these studies.
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