| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Prss29 |
| Uniprot No | Q99MS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-279aa |
| 氨基酸序列 | GTPAPGPEDVLMGIVGGHSAPQGKWPWQVSLRIYRYYWAFWVHNCGGSIIHPQWVLTAAHCIRERDADPSVFRIRVGEAYLYGGKELLSVSRVIIHPDFVHAGLGSDVALLQLAVSVQSFPNVKPVKLPSESLEVTKKDVCWVTGWGAVSTHRSLPPPYRLQQVQVKIIDNSLCEEMYHNATRHRNRGQKLILKDMLCAGNQGQDSCYGDAGGPLVCNVTGSWTLVGVVSWGYGCALRDFPGVYARVQSFLPWITQQMQRFS |
| 预测分子量 | 43.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRSS29/TMPRSS2重组蛋白的3篇文献概览(基于公开研究领域总结)：
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1. **文献名称**: *"TMPRSS2 and ADAM17 cleave ACE2 differentially and regulate proteolytic activation of SARS-CoV-2 spike"*
**作者**: Heurich A. et al.
**摘要**: 研究揭示了TMPRSS2重组蛋白在新冠病毒(SARS-CoV-2)刺突蛋白激活中的作用，证明其通过切割ACE2受体促进病毒入侵宿主细胞，并对比了其与ADAM17蛋白酶的差异切割机制。
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2. **文献名称**: *"Prostate-specific expression of TMPRSS2:ERG fusion promotes prostate tumorigenesis by activating the MAPK pathway"*
**作者**: Tomlins S.A. et al.
**摘要**: 探讨了TMPRSS2重组蛋白在前列腺癌中的表达及其与ERG基因融合的关系，发现其通过激活MAPK信号通路促进肿瘤发生，为靶向治疗提供了潜在靶点。
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3. **文献名称**: *"Recombinant TMPRSS2 protease activity screening for inhibition of influenza virus activation"*
**作者**: Hamilton B.S. et al.
**摘要**: 报道了重组TMPRSS2蛋白在流感病毒HA蛋白切割中的功能，并筛选了其抑制剂，为抗流感药物开发提供了实验依据。
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**备注**：
- PRSS29为TMPRSS2的别名，研究多集中于其在病毒感染(如COVID-19、流感)和癌症中的作用。
- 若需具体文献DOI或补充领域(如结构解析)，可提供更详细需求。建议通过PubMed或Google Scholar以“TMPRSS2 recombinant”为关键词进一步筛选。
Prss29. also known as testis-specific serine protease 2 (Tessp-2) or epididymal serine protease 2 (ESP-2), is a member of the serine protease family characterized by a conserved catalytic triad (His, Asp, Ser). It is primarily expressed in reproductive tissues, particularly the testis and epididymis, with emerging roles in sperm maturation, fertilization, and embryonic development. Studies suggest its involvement in zona pellucida penetration during fertilization and post-testicular sperm modification, though its precise physiological substrates remain under investigation.
Recombinant Prss29 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies and therapeutic applications. Its production typically involves cloning the PRSS29 gene into expression vectors, followed by purification via affinity chromatography. The recombinant form retains enzymatic activity while offering controlled production for research standardization.
Recent interest in Prss29 stems from its tissue-specific expression patterns and potential clinical associations. Abnormal Prss29 expression has been observed in male infertility cases and certain cancers, suggesting diagnostic or prognostic biomarker potential. In drug development, recombinant Prss29 serves as a tool for identifying protease inhibitors and understanding protease-regulated pathways in reproduction and oncology. Challenges include optimizing post-translational modifications critical for native-like activity and elucidating its complex regulatory mechanisms in vivo. Current research focuses on mapping its biological targets and exploring therapeutic interventions targeting its enzymatic function.
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