| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2BN |
| Uniprot No | Q99877 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-126aa |
| 氨基酸序列 | PEPSKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK |
| 预测分子量 | 29.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H2BN重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，非真实文献)：
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1. **文献名称**：*Efficient Expression and Purification of Recombinant HIST1H2BN in E. coli*
**作者**：Zhang W, et al.
**摘要**：研究报道了在大肠杆菌系统中优化表达HIST1H2BN重组蛋白的方法，通过His标签亲和层析和凝胶过滤纯化获得高纯度蛋白，验证其用于体外核小体组装的功能活性。
2. **文献名称**：*HIST1H2BN Modulates Chromatin Accessibility in Gene Regulation*
**作者**：Smith JL, Brown K
**摘要**：利用重组HIST1H2BN蛋白进行染色质免疫沉淀测序(ChIP-seq)，发现其通过特定乙酰化修饰影响染色质开放状态，参与调控细胞分化相关基因的表达。
3. **文献名称**：*Structural Insights into HIST1H2BN via Cryo-EM Analysis*
**作者**：Garcia R, et al.
**摘要**：通过冷冻电镜解析了重组HIST1H2BN在核小体中的高分辨率结构，揭示了其N端尾部构象的动态变化对DNA-组蛋白相互作用的影响。
4. **文献名称**：*HIST1H2BN Dysregulation in Breast Cancer Progression*
**作者**：Li Y, Chen X
**摘要**：研究利用重组HIST1H2BN蛋白制备多克隆抗体，发现其在乳腺癌组织中表达显著下调，并通过体外实验证明其过表达抑制肿瘤细胞迁移。
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**建议**：如需真实文献，推荐在PubMed或Google Scholar中检索关键词“HIST1H2BN recombinant protein”或“H2B histone family expression”，筛选近五年研究以获取最新进展。
**Background of HIST1H2BN Recombinant Protein**
HIST1H2BN is a member of the histone H2B family, which plays a fundamental role in DNA packaging and chromatin organization. Histones are highly conserved proteins that compact eukaryotic DNA into nucleosomes, the repeating structural units of chromatin. Each nucleosome consists of an octamer of core histones (H2A, H2B, H3. and H4) wrapped by DNA. As a replication-dependent histone, HIST1H2BN is encoded by the HIST1 cluster, located on chromosome 6 (6p22.1), which contains multiple histone genes regulated in a cell cycle-dependent manner.
The H2B family, including HIST1H2BN, contributes to nucleosome stability and serves as a platform for post-translational modifications (e.g., acetylation, methylation, ubiquitination) that regulate chromatin dynamics, gene expression, and epigenetic inheritance. HIST1H2BN is particularly involved in transcriptional regulation, DNA repair, and cellular differentiation. Dysregulation of histone H2B variants has been linked to developmental disorders, cancer, and other diseases, underscoring their biological and clinical relevance.
Recombinant HIST1H2BN protein is produced in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical and functional properties. This engineered protein retains structural and functional features of native H2B, enabling researchers to investigate its interactions with DNA, other histones, and chromatin-modifying enzymes. It is widely used in assays such as chromatin immunoprecipitation (ChIP), nucleosome reconstitution, and studies exploring histone post-translational modifications.
The availability of recombinant HIST1H2BN facilitates mechanistic insights into epigenetic regulation and provides a tool for drug discovery targeting chromatin-related pathways. Its applications span basic research in epigenetics, cancer biology, and therapeutic development.
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