| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPYSL5 |
| Uniprot No | Q9BPU6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-564aa |
| 氨基酸序列 | MLANSASVRILIKGGKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKVIDATGKLVIPGGIDTSTHFHQTFMNATCVDDFYHGTKAALVGGTTMIIGHVLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPKVKAEMETLVREKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAEGAKEALDLGITGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSGVQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRSSGIW |
| 预测分子量 | 66.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPYSL5重组蛋白的3篇参考文献示例(部分信息为模拟虚构，供参考)：
1. **文献名称**：*"Recombinant DPYSL5 promotes axonal growth via CRMP signaling in neuronal cells"*
**作者**：Saito T, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组DPYSL5蛋白，发现其通过激活CRMP信号通路显著促进体外培养的神经元轴突生长，提示其在神经再生中的潜在应用价值。
2. **文献名称**：*"Structural and functional characterization of human DPYSL5 recombinant protein"*
**作者**：Wang L, et al.
**摘要**：通过X射线晶体学解析了重组DPYSL5蛋白的三维结构，并验证其与微管蛋白的结合能力，揭示了DPYSL5在细胞骨架重塑中的分子机制。
3. **文献名称**：*"DPYSL5 as a biomarker in glioma: Recombinant protein-based clinical analysis"*
**作者**：Chen H, et al.
**摘要**：利用昆虫细胞系统制备高纯度DPYSL5重组蛋白，开发ELISA检测方法，发现其在胶质瘤患者血清中表达上调，可能作为肿瘤诊断的生物标志物。
**备注**：若需真实文献，建议通过PubMed或Google Scholar搜索关键词“DPYSL5 recombinant protein”获取最新研究。部分研究可能侧重于DPYSL5的功能而非重组制备，需结合摘要筛选。
**Background of DPYSL5 Recombinant Protein**
Dihydropyrimidinase-like 5 (DPYSL5), also known as Collapsin Response Mediator Protein 5 (CRMP5), is a member of the DPYSL/CRMP family, which plays critical roles in neuronal development, axonal guidance, and synaptic plasticity. These proteins are involved in cytoskeletal dynamics by regulating microtubule assembly and actin polymerization, processes essential for neurite outgrowth, growth cone collapse, and neuronal migration. DPYSL5 is particularly expressed in the nervous system and has been implicated in neurodevelopmental disorders, neurodegenerative diseases, and cancer progression.
Structurally, DPYSL5 contains conserved domains common to CRMPs, including a GST-like N-terminal domain and a C-terminal helical domain, which mediate interactions with tubulin heterodimers and other signaling molecules. Dysregulation of DPYSL5 expression or function has been linked to pathological conditions. For example, reduced DPYSL5 levels are observed in Alzheimer’s disease, while its overexpression in certain cancers correlates with metastasis and poor prognosis.
Recombinant DPYSL5 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling high-purity, functional protein for research. It serves as a tool to study molecular mechanisms underlying neuronal development, signal transduction (e.g., Semaphorin, Wnt pathways), and disease pathogenesis. Additionally, recombinant DPYSL5 is used in drug screening, antibody development, and as a biomarker in diagnostic assays. Its role in modulating cytoskeletal organization and cell motility also makes it relevant in cancer biology and regenerative medicine research.
Overall, DPYSL5 recombinant protein is a vital resource for dissecting its physiological and pathological roles, offering potential therapeutic insights for neurological and oncological disorders.
×
