| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | enoA |
| Uniprot No | P06733 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK |
| 预测分子量 | 47,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于enoA重组蛋白的3篇参考文献摘要信息：
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1. **标题**: *Cloning, expression, and characterization of enoA gene in Streptococcus suis*
**作者**: Li Y, et al.
**摘要**: 研究成功克隆并表达了猪链球菌中的enoA基因，重组蛋白在大肠杆菌中高效表达，并通过His标签纯化。酶活性分析证实重组EnoA具有烯醇化酶功能，为后续疫苗开发奠定基础。
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2. **标题**: *Recombinant enoA as a potential vaccine candidate against Enterococcus faecalis infections*
**作者**: Wang H, et al.
**摘要**: 通过原核系统表达粪肠球菌EnoA重组蛋白，动物实验显示其能诱导强烈的体液免疫反应，显著提高小鼠对细菌感染的存活率，提示其作为疫苗抗原的潜力。
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3. **标题**: *Structural and functional analysis of enolase from Bacillus subtilis*
**作者**: Zhang L, et al.
**摘要**: 解析枯草芽孢杆菌EnoA重组蛋白的晶体结构，揭示其底物结合位点及催化机制。体外实验验证其糖酵解功能，并发现其可能参与细菌生物膜形成。
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注：以上文献信息为示例，实际研究中建议通过PubMed或Google Scholar以“enoA recombinant protein”等关键词检索最新文献。
Enolase A (EnoA) is a highly conserved glycolytic enzyme that catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate, a critical step in glycolysis. Beyond its metabolic role, EnoA exhibits "moonlighting" functions, including interactions with host proteins, involvement in pathogenicity, and immune evasion, particularly in bacterial species like *Streptococcus* and *Staphylococcus*. For instance, in *Streptococcus pneumoniae*, EnoA acts as a surface plasminogen receptor, facilitating tissue invasion and immune system evasion.
Recombinant EnoA proteins are engineered via cloning the *enoA* gene into expression vectors (e.g., *E. coli* or yeast systems) for large-scale production. This enables studies on its structural and functional diversity across species. Researchers utilize recombinant EnoA to investigate its dual roles in metabolism and virulence, aiding vaccine development and therapeutic targeting. In diagnostics, EnoA serves as an antigen for detecting bacterial infections.
Recent studies highlight its potential as a vaccine candidate due to its surface exposure and immunogenicity. Structural analyses of recombinant EnoA also provide insights into enzyme mechanics and inhibitor design. Challenges include optimizing solubility and post-translational modifications in heterologous systems. Overall, EnoA exemplifies the intersection of microbial metabolism and pathogenicity, making it a versatile target in biomedical research.
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