| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PNKP |
| Uniprot No | Q96T60 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521aa |
| 氨基酸序列 | MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQ VELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLH PLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENL EKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIP RKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVL VATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPG RKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSG PLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLG SWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLF TATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILE IPFRLWVEPRLGRLYCQFSEG |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PNKP重组蛋白的3-4条参考文献示例(内容基于公开文献概括，具体细节需以实际论文为准)：
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1. **文献名称**：*Structural and functional characterization of human polynucleotide kinase/phosphatase*
**作者**：Mani, R.S., et al.
**摘要**：该研究报道了人源PNKP重组蛋白的克隆、表达及功能分析，揭示了其双功能酶活性(5'-激酶和3'-磷酸酶)在DNA断口修复中的协同作用，并探讨了其与XRCC1蛋白的相互作用机制。
2. **文献名称**：*Crystal structure of human polynucleotide kinase/phosphatase reveals insight into substrate specificity and DNA repair*
**作者**：Dahan, D., et al.
**摘要**：通过X射线晶体学解析了PNKP重组蛋白的分子结构，阐明了其底物结合口袋的构象特征，解释了其对DNA末端不同化学基团的选择性催化机制，为设计靶向抑制剂提供了结构基础。
3. **文献名称**：*PNKP mutations associated with neurodegenerative disorders impair enzymatic activity and compromise DNA repair*
**作者**：Shen, J., et al.
**摘要**：研究利用重组PNKP蛋白进行突变体功能分析，发现某些与遗传性神经疾病相关的突变(如微头畸形相关突变)显著降低其激酶/磷酸酶活性，导致DNA单链断裂修复缺陷和细胞凋亡增加。
4. **文献名称**：*Enhancing CRISPR-Cas9 editing efficiency by co-expression of PNKP for DNA end repair*
**作者**：Wang, H., et al.
**摘要**：该文献探讨了重组PNKP蛋白在基因编辑中的应用，证明其与CRISPR-Cas9系统共表达可有效修复DNA末端损伤，提高同源重组修复效率，为精准基因治疗提供了新策略。
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如需具体文献来源，建议通过PubMed或Google Scholar搜索上述关键词获取全文。
PNKP (Polynucleotide Kinase/Phosphatase) is a bifunctional enzyme critical for repairing DNA damage, particularly single-strand breaks (SSBs) and double-strand breaks (DSBs). It plays a pivotal role in the DNA damage response by restoring genomic integrity through two opposing enzymatic activities: a kinase domain that phosphorylates 5′-hydroxyl ends and a phosphatase domain that dephosphorylates 3′-phosphate termini. This dual functionality ensures proper end compatibility for subsequent ligation during repair processes.
PNKP is essential in multiple DNA repair pathways, including base excision repair (BER) and non-homologous end joining (NHEJ). It interacts with key repair proteins such as XRCC1 and XRCC4. acting as a scaffold to coordinate repair steps. Mutations in PNKP are linked to severe human disorders, including microcephaly, seizures, and neurodegenerative diseases like ataxia-oculomotor apraxia syndrome, underscoring its importance in neuronal homeostasis.
Recombinant PNKP proteins, produced via expression systems like *E. coli* or insect cells, retain native enzymatic activities and are widely used to study DNA repair mechanisms *in vitro*. These proteins enable biochemical assays to dissect kinase/phosphatase kinetics, screen inhibitors for therapeutic development, or analyze structural features via crystallography. Additionally, recombinant PNKP aids in modeling disease-associated mutations, offering insights into their functional impact. Its role as a potential therapeutic target in cancer, where DNA repair pathways are often dysregulated, further drives research using recombinant forms to explore precision oncology strategies. Overall, PNKP recombinant tools are indispensable for advancing both basic and translational studies in genome stability and disease.
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