| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEKT5 |
| Uniprot No | Q96M29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-485aa |
| 氨基酸序列 | MEFLGTTQTASYCGPKKCCGLTSLPAVQAPVIQECYQPYYLPGYRYLNSWRPSLFYKIANVQTCPDESTSTLRPPTILPTLRSALFSRYSPHDWDQSNQLQVRGAEASRLWASRLTDDSMRLLQDKDQLTHQMQEGTCRNLGQRLSDIGFWKSELSYELDRLLTENQNLETVKRRLECAANEVNCPLQVALECLYHREKRIGIDLVHDNVEKNLIREVDLLKCCQEQMRKLAQRIDIQMRDNRDAQHVLERDLEDKSSAQCIDEKCFNLRNTSDCISFFHGMEKIDGTISVPETWAKFSNDNIKHSQNMRANSIQLREEAEHLFETLSDQMWRQFTDTNLAFNARISEVTDVKNKLQTQLAKTLQEIFQAENTIMLLERSIMAKEGPLKVAQTRLECRTRRPNMELCRDIPQLKLVNEVFTIDDTLQTLKLRLRETQDTLQLLVMTKCRLEHELAIKANTLCIDKEKCMGMRKTFPCTPRLVGHT |
| 预测分子量 | 72.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TEKT5重组蛋白的3篇参考文献示例(内容基于虚构文献概括，仅供参考)：
1. **文献名称**：*重组TEKT5蛋白的克隆表达及其在精子鞭毛结构中的功能分析*
**作者**：Zhang L, Wang Y, Chen H
**摘要**：本研究通过基因工程技术在大肠杆菌中成功表达并纯化了TEKT5重组蛋白，证实其能够与微管蛋白相互作用，并参与精子鞭毛的稳定性调控。实验表明，TEKT5的缺失会导致鞭毛结构异常，影响精子运动能力。
2. **文献名称**：*TEKT5重组蛋白的晶体结构解析及其与不育症的关联研究*
**作者**：Smith J, Kumar R, Tanaka K
**摘要**：作者利用X射线晶体学首次解析了人类TEKT5重组蛋白的三维结构，揭示了其螺旋结构域的关键功能位点。进一步临床样本分析表明，TEKT5突变可能通过破坏蛋白二聚化导致男性不育。
3. **文献名称**：*基于TEKT5重组蛋白的体外自组装模型构建*
**作者**：Garcia M, Lee S, Patel D
**摘要**：该研究通过重组TEKT5蛋白在体外模拟了纤毛内骨架的自组装过程，发现其与TEKT2蛋白存在协同作用。实验结果为理解纤毛发生机制及相关疾病提供了分子基础。
(注：以上文献为示例性内容，实际研究中请通过学术数据库检索真实发表的论文。)
**Background of TEKT5 Recombinant Protein**
TEKT5 (tektin-5) is a member of the tektin family, a group of evolutionarily conserved proteins primarily associated with microtubule-based structures in cilia, flagella, and centrioles. These proteins play critical roles in maintaining the structural integrity and functional dynamics of cytoskeletal networks. Specifically, TEKT5 is expressed in germ cells and is implicated in spermatogenesis, where it contributes to the stability and motility of sperm flagella. Its dysfunction has been linked to male infertility, highlighting its importance in reproductive biology.
Recombinant TEKT5 protein is produced through genetic engineering techniques, typically by inserting the TEKT5 gene into expression vectors (e.g., bacterial, insect, or mammalian systems) to enable large-scale production. This approach ensures high purity and specificity, allowing researchers to study TEKT5's biochemical properties, interactions, and role in cellular processes. The recombinant form retains functional domains essential for its interaction with microtubules and other tektin family members.
Studies utilizing TEKT5 recombinant protein have advanced understanding of ciliopathies, reproductive disorders, and cellular mechanotransduction. It serves as a tool for investigating molecular mechanisms underlying sperm motility defects and ciliary dysfunctions. Additionally, TEKT5 has potential applications in developing diagnostic markers for infertility or therapeutic strategies targeting microtubule-related pathologies.
Research on TEKT5 also extends to cancer biology, as altered expression of tektins has been observed in certain malignancies, suggesting roles in cell division or metastasis. Overall, TEKT5 recombinant protein is a vital resource for dissecting the molecular basis of both physiological and disease-associated processes involving microtubule networks.
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