| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EME1 |
| Uniprot No | Q96AY2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-583aa |
| 氨基酸序列 | MALKKSSPSLDSGDSDSEELPTFAFLKKEPSSTKRRQPEREEKIVVVDISDCEASCPPAPELFSPPVPEIAETVTQTQPVRLLSSESEDEEEFIPLAQRLTCKFLTHKQLSPEDSSSPVKSVLDHQNNEGASCDWKKPFPKIPEVPLHDTPERSAADNKDLILDPCCQLPAYLSTCPGQSSSLAVTKTNSDILPPQKKTKPSQKVQGRGSHGCRQQRQARQKESTLRRQERKNAALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD |
| 预测分子量 | 80.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EME1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Structure and function of the MUS81-EME1 complex in DNA repair"*
**作者**: Matos, J., West, S.C.
**摘要**: 该研究解析了MUS81-EME1重组蛋白复合物的晶体结构，揭示其如何特异性识别并切割DNA交叉结构，阐明了其在DNA损伤修复中的分子机制。
2. **文献名称**: *"Recombinant EME1 enhances Holliday junction resolution by MUS81 in vitro"*
**作者**: Ciccia, A., Constantinou, A., et al.
**摘要**: 通过体外实验证明重组表达的EME1蛋白与MUS81协同作用，显著提升对Holliday连接体的切割效率，强调了EME1在维持基因组稳定性中的关键作用。
3. **文献名称**: *"Functional characterization of EME1 in meiotic recombination"*
**作者**: Holloway, J.K., Booth, J., et al.
**摘要**: 利用重组EME1蛋白进行功能分析，发现其在减数分裂过程中通过调控重组中间体的解离，确保染色体正确分离，缺陷会导致生殖细胞异常。
以上文献均聚焦于EME1重组蛋白的结构、功能及其在DNA修复或减数分裂中的机制研究。如需具体期刊信息或发表年份，可进一步补充检索条件。
EME1 (Essential Meiotic Structure-Specific Endonuclease subunit 1) is a critical protein involved in DNA repair and genomic stability, primarily functioning as a regulatory subunit of the MUS81-EME1 heterodimeric endonuclease complex. This complex plays a key role in resolving DNA recombination intermediates, such as stalled replication forks, Holliday junctions, and DNA crosslinks, during homologous recombination repair (HRR) and meiosis. EME1 stabilizes MUS81 and enhances its enzymatic activity, enabling precise cleavage of branched DNA structures to facilitate error-free repair. Dysregulation of EME1 is linked to genomic instability, chromosomal abnormalities, and cancer susceptibility.
Recombinant EME1 protein is produced using biotechnological methods, typically through expression in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by affinity purification. Its recombinant form retains the structural and functional properties of native EME1. making it a valuable tool for *in vitro* studies. Researchers use it to investigate DNA repair mechanisms, characterize interactions with MUS81 or other partners, and screen for compounds modulating nuclease activity. Additionally, it aids in studying the molecular basis of diseases associated with defective DNA repair, such as Fanconi anemia or BRCA-mutant cancers.
Studies involving recombinant EME1 have advanced our understanding of chemotherapy resistance mechanisms, as tumor cells with impaired MUS81-EME1 activity often show reduced sensitivity to DNA-damaging agents like cisplatin. Ongoing research aims to exploit this pathway for targeted cancer therapies, highlighting EME1's dual role as a genomic caretaker and a potential therapeutic vulnerability.
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