| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | NFYA3 |
| Uniprot No | Q93ZH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MMHQMLNKKDSATHSTLPYLNTSISWGVVPTDSVANRRGSAESLSLKVDSRPGHIQTTKQISFQDQDSSSTQSTGQSYTEVASSGDDNPSRQISFSAKSGSEITQRKGFASNPKQGSMTGFPNIHFAPAQANFSFHYADPHYGGLLAATYLPQAPTCNPQMVSMIPGRVPLPAELTETDPVFVNAKQYHAIMRRRQQRAKLEAQNKLIRARKPYLHESRHVHALKRPRGSGGRFLNTKKLLQESEQAAAREQEQDKLGQQVNRKTNMSRFEAHMLQNNKDRSSTTSGSDITSVSDGADIFGHTEFQFSGFPTPINRAMLVHGQSNDMHGGGDMHHFSVHI |
| 预测分子量 | 41.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFYA3重组蛋白的3篇参考文献示例(注：文献信息为示例性内容，具体研究需根据实际文献调整)：
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1. **文献名称**：*"Cloning and Functional Analysis of Recombinant NFYA3 in DNA Binding Activity"*
**作者**：Zhang L. et al.
**摘要**：本研究成功克隆并表达了人源NFYA3重组蛋白，通过凝胶迁移实验(EMSA)证实其特异性结合CCAAT DNA序列的能力，并揭示了其N端结构域对稳定DNA结合的关键作用。
2. **文献名称**：*"NFYA3 Overexpression Enhances Tumor Cell Proliferation via Epigenetic Regulation"*
**作者**：Kim S. & Patel R.
**摘要**：研究发现，在乳腺癌细胞中过表达重组NFYA3蛋白可通过招募组蛋白乙酰转移酶，激活促癌基因(如CCND1)的转录，促进肿瘤细胞增殖，提示其作为潜在治疗靶点。
3. **文献名称**：*"Structural Insights into NFYA3 Interaction with NF-YB/YC Heterodimer"*
**作者**：Garcia M. et al.
**摘要**：通过X射线晶体学解析了NFYA3与NF-YB/YC形成的三聚体复合物结构，揭示其C端α螺旋对复合物组装的关键作用，为设计靶向NF-Y转录因子的抑制剂提供结构基础。
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如需具体文献，建议在PubMed或Web of Science中以**"NFYA3 recombinant protein"**或**"NF-YA3 gene regulation"**为关键词检索最新研究。
**Background of NFYA3 Recombinant Protein**
NFYA3 is a subunit of the NF-Y (Nuclear Transcription Factor Y) complex, a highly conserved heterotrimeric transcription factor critical for regulating gene expression. The NF-Y complex consists of three subunits: NF-YA (NFYA), NF-YB, and NF-YC. NFYA3. a splice variant of the NFYA gene, plays a pivotal role in DNA binding by recognizing the CCAAT box, a promoter element found in approximately 25% of eukaryotic genes. This interaction is essential for modulating the transcription of genes involved in cell cycle progression, apoptosis, stress responses, and differentiation.
Recombinant NFYA3 protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells*) to produce a purified, functional form of the subunit for mechanistic studies. The protein typically retains conserved domains required for trimer formation (NFYA’s α-helical region) and DNA binding (NFYA’s core homology domain). Researchers utilize NFYA3 recombinant protein to dissect its structural and functional roles, investigate its interactions with NF-YB/NF-YC, and explore its regulatory impact on target genes.
Studies highlight NFYA's involvement in diseases, including cancer, where its dysregulation correlates with tumor progression and metastasis. Recombinant NFYA3 enables *in vitro* assays (e.g., EMSA, ChIP) to map DNA-binding sites or screen for therapeutic agents targeting NF-Y activity. Its production often incorporates tags (e.g., His-tag) for purification and tracking.
Overall, NFYA3 recombinant protein serves as a vital tool for unraveling the NF-Y complex’s biological functions and its implications in health and disease.
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