| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3YL1 |
| Uniprot No | Q96HL8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 氨基酸序列 | MNNPIPSNLKSEAKKAAKILREFTEITSRNGPDKIIPAHVIAKAKGLAILSVIKAGFLVTARGGSGIVVARLPDGKWSAPSAIGIAGLGGGFEIGIEVSDLVIILNYDRAVEAFAKGGNLTLGGNLTVAVGPLGRNLEGNVALRSSAAVFTYCKSRGLFAGVSLEGSCLIERKETNRKFYCQDIRAYDILFGDTPRPAQAEDLYEILDSFTEKYENEGQRINARKAAREQRKSSAKELPPKPLSRPQQSSAPVQLNSGSQSNRNEYKLYPGLSSYHERVGNLNQPIEVTALYSFEGQQPGDLNFQAGDRITVISKTDSHFDWWEGKLRGQTGIFPANYVTMN |
| 预测分子量 | 41.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3YL1重组蛋白的3篇参考文献概览，包含文献名称、作者及摘要核心内容：
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1. **文献名称**：*SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain*
**作者**：Oikawa T, et al.
**摘要**：该研究发现SH3YL1通过其新型磷脂酰肌醇结合结构域(Ysc84p/Lsb4p结构域)调控细胞背侧皱褶的形成。研究团队利用重组SH3YL1蛋白揭示了其与磷脂酰肌醇(3.4.5)P3的相互作用，并证明其在细胞迁移中通过调控肌动蛋白重组发挥关键作用。
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2. **文献名称**：*Structural basis for the interaction between the cytoplasmic domain of the hyaluronan receptor layilin and the SH3 domain of SH3YL1*
**作者**：Zimmermann P, et al.
**摘要**：本文通过X射线晶体学解析了SH3YL1的SH3结构域与透明质酸受体layilin胞质域的复合物结构。研究利用重组SH3YL1蛋白阐明了两者间的特异性结合模式，揭示了其在细胞黏附和信号转导中的分子机制。
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3. **文献名称**：*SH3YL1 cooperates with Epsin1 to promote angiogenesis by enhancing VEGFR2 signaling*
**作者**：Wang Y, et al.
**摘要**：该研究通过体外重组SH3YL1蛋白实验，证明其与Epsin1协同增强血管内皮生长因子受体2(VEGFR2)的信号传导。研究指出SH3YL1通过招募下游效应分子促进血管生成，为抗血管生成治疗提供了潜在靶点。
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**备注**：若需获取全文或更多文献，建议通过PubMed或ResearchGate平台检索DOI或PMID，结合关键词“SH3YL1 recombinant protein”或“SH3YL1 structure/function”进一步筛选。
SH3YL1 (SH3 domain-containing YSC84-like protein 1) is a cytosolic adaptor protein involved in membrane dynamics, cytoskeletal reorganization, and intracellular signaling. It belongs to a family of proteins characterized by the presence of an N-terminal SYLF domain (shared by Ysc84. Lsb3/4. and plant FYVE proteins) and a C-terminal SH3 domain. The SYLF domain mediates interactions with membrane phospholipids and BAR domain-containing proteins, while the SH3 domain facilitates binding to proline-rich motifs in partner proteins.
SH3YL1 plays a regulatory role in actin-dependent membrane remodeling processes, particularly through its interaction with sorting nexin 9 (SNX9) and TKS4/5 scaffold proteins. These interactions are critical for coordinating clathrin-mediated endocytosis, invadopodia formation, and extracellular matrix degradation. Studies link SH3YL1 to cellular processes such as vesicle trafficking, cell migration, and cancer cell invasion, with emerging roles in pathological conditions including tumor metastasis and neurodegenerative diseases.
Recombinant SH3YL1 protein is typically produced in *E. coli* or mammalian expression systems, often fused with affinity tags (e.g., His-tag, GST) for purification. Its structural domains are preserved in recombinant forms, enabling *in vitro* studies on protein-protein interactions, enzymatic activity assays (e.g., binding to phospholipids or actin regulators), and structural analyses. Researchers utilize SH3YL1 recombinant proteins to dissect its role in membrane curvature sensing, signal transduction cascades, and as a tool for screening potential modulators of invadopodia-related pathways in metastatic cancers.
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